对电子烟组件的急性暴露会改变骨质前细胞的mRNA表达
Shareef M Dabdoub1,2, Ashley Greenlee3, George Abboud4
1Division of Biostatistics and Computational Biology, College of Dentistry, University of Iowa, Iowa City, Iowa, USA.
概括
电子尼古丁输送系统 (ENDS) 组件,尼古丁和载体液体 (PG/VG),改变面细胞循环信号和应激反应. 这些发现表明影响骨发育的协同效应,需要进一步研究ENDS配方安全性.
科学领域:
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
- 基因组学就是基因组学.
背景情况:
- 传统的烟草使用与不良健康结果有关.
- 电子尼古丁输送系统 (ENDS) 引发了新的健康问题.
- 关于ENDS组件,如尼古丁和载体液体 (PG/VG) 的特定健康影响的研究有限.
研究的目的:
- 为了研究尼古丁和常见的ENDS载体成分 (propylene glycol/植物甘油) 对骨质前细胞的体外影响.
- 探索与传统烟草产品相比,对面骨的潜在协同健康影响.
- 为了确定受ENDS组件暴露影响的分子标和途径.
主要方法:
- 鼠类MC3T3-E1骨质前细胞被培养并暴露在尼古丁,PG/VG,尼古丁+PG/VG和商用vape液体 (Juul) 中.
- 用RNA测序 (RNA-Seq) 来分析急性24小时治疗后的mRNA信号变化.
- 稀疏的部分最小方程区分分析 (sPLS-DA) 和PANTHER分类用于基因标识和途径分析. 评估了细胞周期和自标志物.
主要成果:
- 暴露于尼古丁+PG/VG显著改变了骨质前细胞周期信号,影响细胞数量并诱导细胞应激 (自标志物),而不会增加亡.
- sPLS-DA揭示了基于暴露类型的明显分离模式,突出尼古丁对细胞粘附,骨质细胞分化和新陈代谢的影响,以及PG/VG在细胞迁移和生存中的作用.
- 基因共同表达网络分析确定了面骨发育中的关键途径 (FGF,Notch,TGFβ,WNT) 受到ENDS暴露的影响,其中一些途径显示Juul暴露的影响更大.
结论:
- 在ENDS配方中同时暴露于尼古丁和载体液体会导致骨质前细胞周期和应激反应的显著改变.
- 尼古丁和PG/VG之间有明显的协同效应,影响了对面骨发育至关重要的分子通路.
- 这些发现强调需要进一步调查ENDS配方安全性及其潜在的长期健康影响.
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