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重定向MAP激酶信号,以形成掠食性酵母酵母中的透
Mareike Rij1, Yeseren Kayacan2, Beatrice Bernardi1,2
1Department of Microbiology and Biochemistry, Hochschule Geisenheim University, Geisenheim, Germany.
PLoS pathogens
|August 30, 2024
概括
MAP激酶信号通路调节了Saccharomycopsis酵母中的掠夺行为,使它们能够形成专门的结构来攻击其他细胞. 这条通路对于它们独特的真菌寄生虫生活方式至关重要.
科学领域:
- * 菌群学 * 菌群学
- * 分子生物学 * 分子生物学
- * 进化生物学 进化生物学
背景情况:
- * 赛卡罗米科普西斯酵母在异菌性酵母中是独一无二的,因为它们具有天然的硫辅性.
- * 甲氨酸饥饿引发了转向掠食性,真菌寄生虫的生命策略.
- *这种掠夺性行为涉及形成富含基的透钉,用于猎物入侵,类似于真菌植物病原体.
研究的目的:
- * 调查MAP激酶信号通路在调节Saccharomycopsis schoenii中的透形成和掠食中的作用.
- * 确定参与酵母捕食生活方式的关键基因和调控元素.
主要方法:
- *基因删除实验以创造缺乏SsKIL1 (MAP激酶) 或SsSTE12 (转录因子) 的突变物.
- *使用RNA测序 (RNAseq) 进行比较的全球转录组分析,以比较基因表达特征.
- * 促进子序列的分析,以确定cis-regulatory元素.
主要成果:
- *删除SsKIL1或SsSTE12导致非致病突变不能形成透钉.
- *RNAseq分析显示,SsKil1-SsSte12依赖的捕食反应丧失,而 metionin饥饿反应保持完整.
- * 一个类似于Saccharomyces cerevisiae Ste12激素反应元素的cis-regulatory元素,在掠食过程中调高的基因中被确定.
结论:
- * 古代和保存的MAP激酶信号通路,特别是SsKil1-SsSte12模块,调节了S. schoenii的透形成和掠食.
- *通过MAP激酶信号传递调节的Ste12转录控制对掠食特异性基因的重新连接,可能有助于酵母中掠食性行为的并行进化.
- *在这些酵母菌种中,SSSTE12对于交配是不可或缺的.
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