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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

736
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
736
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

4.7K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass.  One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
4.7K
High-Resolution Mass Spectrometry (HRMS)01:15

High-Resolution Mass Spectrometry (HRMS)

1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

1.1K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.1K

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相关实验视频

Updated: Jun 14, 2025

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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pyMS-Vis,一个开源的Python应用程序,用于可视化和调查解密的自上而下的质谱实验:一个Histone Proteoform案例研究.

James J Pesavento1, Megan S Bindra1, Udayan Das1

  • 1Saint Mary's College of California, Moraga, California 94575, United States.

Analytical chemistry
|August 30, 2024
PubMed
概括

我们开发了一个开源的Python应用程序,用于分析液态染色学上下质谱 (LC-TDMS) 数据. 这种工具量化了蛋白质形式的丰富性,揭示了对Chlamydomonas reinhardtii. histone修饰的新见解.

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Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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相关实验视频

Last Updated: Jun 14, 2025

Isolation of Histone from Sorghum Leaf Tissue for Top Down Mass Spectrometry Profiling of Potential Epigenetic Markers
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Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis
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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物化学 生物化学
  • 计算生物学 计算生物学

背景情况:

  • 顶向下质谱 (TDMS) 对于分析完整的蛋白质及其修改至关重要.
  • 液体染色学与TDMS (LC-TDMS) 结合,可使复杂的蛋白形混合物进行分离和分析.
  • 对蛋白质形式,特别是基因组的定量分析对于理解细胞调节至关重要.

研究的目的:

  • 开发一个开源的Python应用程序,用于对LC-TDMS数据进行定量和定性分析.
  • 为了能够快速准确地量化蛋白质形式的丰度.
  • 为了促进从复杂的蛋白质组数据集中发现新的生物学见解.

主要方法:

  • 开发一个用户友好的Python应用程序,用于处理解密的LC-TDMS数据.
  • 实现搜索有兴趣的质量,可视化离子强度和量化相对丰度的功能.
  • 该软件的应用用于分析来自Chlamydomonas reinhardtii. histone的蛋白质形式.

主要成果:

  • 该应用程序大大缩短了从数小时到几分钟的分析时间,用于蛋白质形式量化.
  • 对Chlamydomonas组织蛋白质蛋白质形式的定量分析揭示了对α-amino termini处理的新见解.
  • 证明了素H2A家族成员中α-氨基终端的独家加工.

结论:

  • 开发的Python应用程序为LC-TDMS数据分析提供了一个高效且易于使用的工具.
  • 该软件能够对蛋白质形态动力学进行定量洞察,促进生物发现.
  • 开源性质促进了可定制性和在质谱学社区内更广泛的采用.