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在侧流和传统增强生物去除 (EBPR) 系统之间,积生物体 (PAO) 的显著微型多样性具有性能影响

Guangyu Li¹, Varun Srinivasan², Nicholas B Tooker²

¹School of Civil and Environmental Engineering, Cornell University, Ithaca, NY, United States; Department of Civil and Environmental Engineering, Northeastern University, Boston, MA, United States.

|August 30, 2024

概括

多积聚生物体 (PAO) 的微生物多样性对于增强的生物去除 (EBPR) 系统至关重要. 寡型化分析揭示了独特的亚属级PAO配置文件,为废水处理中的微生物群落提供了快速,经济高效的分析方法.

科学领域:

环境微生物学环境微生物学
废水处理技术废水处理技术
分子生态学分子生态学

背景情况:

聚酸盐积聚生物 (PAO) 是增强生物去除 (EBPR) 和侧流EBPR (S2EBPR) 系统的核心,影响过程稳定性.
目前用于分析现实世界废水样本中PAO微生物多样性的方法通常是缓慢的,昂贵的或无效的.
了解PAO微生物多样性对于优化废水处理中的去除至关重要.

研究的目的:

开发和应用一种快速,具有成本效益的方法,以解决PAO在亚属层面上的微型多样性.
为了研究关键PAO属的微生物多样性 (Ca. 在不同的废水资源回收设施 (WRRFs) 跨越不同的废水资源回收设施 (Accumulibacter, Tetrasphaera, Comamonas).
为了将PAO微型多样性与EBPR/S2EBPR系统性能相关联.

主要方法:

16S rRNA基因扩增序列测序,然后进行寡型化分析.
在12个WRRF中对PAO寡型特征进行比较分析.
网络分析和寡型形状和性能参数之间的相关性研究.

主要成果:

寡型化成功地解决了亚属水平的PAO微生物多样性,超过了传统16SrRNA测序的分辨率.
在不同的WRRF中观察到明显的PAO寡型形状,设施级别的差异超过时间变化.
鉴定出来的小类型与已知的PAO类型的遗传学关联证实了该方法的有效性.
网络和相关性分析揭示了PAO物种之间与去除效率相关的潜在功能差异.

结论:

寡型化提供了一种快速且具有成本效益的方法,用于分析活性污泥中PAO微生物多样性.
在亚属层面上,PAO微生物多样性在废水处理设施之间表现出显著的差异,这表明固有系统特定的微生物指纹.
PAO微型多样性是影响EBPR和S2EBPR系统性能的关键因素,对流程优化有潜在影响.

关键词:

16S rRNA 测序的测序方法这是EBPREBPREBPREBPREBPR 微生物多样性微生物多样性有机型化 (Oligotyping) 是指一种类型.一个PAOPAO的PAO.在S2EBPR中,它是S2EBPR.

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相关概念视频

The Phosphorus Cycle

The Phosphorus Cycle

Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.