乳糖对羊二次毛囊体体外发育的影响
Kíscyla O Andrade1, Alane P O Monte1, Regina L S Silva1
1Federal University of Vale do São Francisco, Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of Vale do São Francisco, Petrolina, PE 56300-990, Brazil.
Animal reproduction science
|August 30, 2024
概括
用0.025μM乳糖补充绵羊二次毛囊培养物显著改善了卵细胞的活力和发育. 这种乳糖度增强了谷氨水平,线粒体活性和介质恢复,同时减少了DNA碎片化.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 生物技术是生物技术.
背景情况:
- 毛囊发育是一个能源密集的过程,需要充足的能量基质.
- 用乳糖等能量来源补充培养基可能会增强卵泡活力和生长.
研究的目的:
- 研究乳糖补充剂对绵羊二次卵泡和卵细胞质量在体外的影响.
- 评估对形态,发育,谷氨酸 (GSH) 度,线粒体活动,DNA碎片化和介质恢复的影响.
主要方法:
- 绵羊的二次毛囊在α-MEM+中培养了18天,乳糖度 (0.025,0.05,0.1μM) 或对照介质的不同.
- 评估了卵泡形态,GSH水平,线粒体活性,DNA碎片化 (TUNEL测定) 和体外成熟 (IVM) 后的介质恢复.
主要成果:
- 0.025微米的乳糖显著增加了形态正常的毛囊 (92.5%与75.55%对照).
- 这种度还提高了GSH水平和线粒体活性,同时减少了TUNEL阳性卵细胞 (9.09%与35.09%对照).
- 在0.025μM的乳糖组中,梅奥斯恢复显著更高 (54.5%与45.5%对照).
结论:
- 补充0.025μM的乳糖可以提高生存率,GSH水平,线粒体活性,以及体外培养的绵羊二次毛囊的介质恢复.
- 乳糖为卵泡细胞提供逐渐的能量,有可能改善IVM和体外受精等辅助生殖技术的可行的卵细胞生产.
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