基于分子的确认,马肉香肠与阿根廷的三病爆发有关
Silvio J Krivokapich1, M Fernanda Degese1, Graciana M Gatti1
1Departamento Parasitología, INEI, ANLIS, "Dr. Carlos G. Malbrán", CABA, Argentina.
International journal of food microbiology
|August 30, 2024
概括
阿根廷的三病爆发与含有猪肉,野猪肉和马肉的生香肠有关. 分子方法在所有情况下都确定了Trichinella spiralis,突出显示了从野生动物传播动物性传染的风险.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 食品安全 食品安全
- 分子生物学分子生物学
背景情况:
- 三病是一种通过未煮熟的肉,主要是猪肉传播的动物传播疾病.
- 野生野生动物的消费构成感染菌感染的风险.
- 最近在阿根廷爆发的疫情促使对肉类来源和寄生虫物种进行了调查.
研究的目的:
- 开发和实施一种分子方法来识别生香肠中的Sus scrofa (猪/野猪) 和Puma concolor (马) 肉.
- 确定在阿根廷疫情中负责人类感染的特异性三菌物种.
- 评估从野生动物,特别是马肉中传播动物感染的风险.
主要方法:
- 在宿主物种中设计用于PCR放大和测序线粒体细胞染色体b基因的特定原料.
- 分析了来自科尔多瓦,布宜诺斯艾利斯和拉潘帕省的三病爆发的香肠样本.
- 进行了Trichinella spp.的分子鉴定. 与疫情相关的食品样本中的幼虫.
主要成果:
- 来自科尔多瓦和布宜诺斯艾利斯疫情的香肠含有猪肉和熊猫肉的混合物.
- 熊猫肉是拉潘帕疫情爆发时发现的香肠中唯一的动物来源.
- 三螺旋菌被证实是所有三个调查的疫情的致病原体.
结论:
- 开发的分子方法成功地在与三病有关的香肠中识别了猪肉和熊猫肉.
- 该研究确定了美洲狮肉作为人类三菌感染的来源,这表明存在显著的动物感染风险.
- 这些发现强调了分子诊断在食品安全和动物传染病公共卫生监测方面的重要性.
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