细胞外囊泡衍生的长非编码RNA通过调解亡和自作用参与了甲血毒性
Yujiao Chen1, Jingyu Wang2, Wei Zhang2
1Prenatal Diagnostic Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China; Department of Occupational Health and Environmental Health, School of Public Health, Capital Medical University, Beijing 100069, China; Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China.
Toxicology and applied pharmacology
|August 30, 2024
概括
携带长非编码RNA (lncRNA) 的细胞外囊泡 (EV) 通过诱导亡和自来调解毒性. 这些EV和它们的lncRNA可以作为暴露于的工人的生物标志物.
科学领域:
- 环境健康 环境健康
- 分子生物学分子生物学
- 毒理学 毒理学 毒理学
背景情况:
- 暴露会导致血液毒性,之前的工作涉及长非编码RNA (lncRNAs) 在诱导的亡和自中.
- 细胞外囊泡 (EVs) 衍生 lncRNAs 在毒性和细胞间通信中的特定作用仍然在很大程度上是未知的.
研究的目的:
- 研究EVs和EVs衍生 lncRNAs在诱导的亡和自的功能.
- 探索EVs衍生 lncRNAs作为暴露的生物标志物的潜力.
主要方法:
- 使用细胞培养模型用1,4-二 (1,4-BQ),的代谢物.
- 量化了亡和自,使用GW4869评估了EV分泌,并对EV进行了lncRNA测序.
- 使用qRT-PCR验证的候选lncRNAs和分析了暴露于的工人的血清EVs.
主要成果:
- 来自1,4-BQ治疗细胞的EV通过PI3K-AKT-mTOR和伴侣介导的自途径增强了亡和自.
- 抑制EV分泌减少了素诱导的亡和自.
- 鉴定并验证了来自1,4-BQ处理细胞的EV中的8个上调的lncRNA,这些EV在暴露工人的血清EV中也升高.
结论:
- 通过转移特定的lncRNAs,EVs在素诱导的血液毒性中促进细胞间通信.
- 来自EVs的lncRNAs是素诱导的亡和自的关键媒介.
- 这些lncRNAs显示为评估暴露个体的风险的潜在生物标志物.
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