由T细胞分泌的Granzyme B参与了小症的抗菌免疫反应
Yi Cao1, Jiansong Zhang1, Ding Wang1
1State Key Laboratory of Estuarine and Coastal Research, School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Fish & shellfish immunology
|August 30, 2024
概括
蒂拉皮亚中的Granzyme B (OnGrB) 对于适应性免疫至关重要,T细胞是主要来源. 这项研究揭示了OnGrBB.
科学领域:
- 免疫学 免疫学 免疫学
- 鱼类免疫学 鱼类免疫学
- 比較免疫學 比較免疫學
背景情况:
- 大酶B对哺乳动物的适应性免疫至关重要,但其在早期脊椎动物中的作用尚不清楚.
- 在teleosts中研究Granzyme B提供了对适应性免疫的演变的见解.
研究的目的:
- 研究尼罗河鱼 (Oreochromis niloticus) 中Granzyme B的功能和表达.
- 探索Granzyme B在蒂拉皮亚适应性免疫反应中的作用.
主要方法:
- 克隆和表征的Oreochromis niloticus B (OnGrB) 的大酶.
- 开发一种小鼠抗OnGrB单克隆抗体 (mAb).
- 在T细胞激活和病原体感染后,体外和体内对OnGrB表达的分析.
主要成果:
- OnGrB表现出保留的功能域,并且在鱼组织中得到广泛表达.
- T细胞是OnGrB产生的主要来源,增加后激活和感染.
- p65初步被确定为OnGrB转录活性的调节者.
结论:
- 在鱼适应性免疫和T细胞功能中,OnGrB起着重要作用.
- 这项研究为远距离适应性免疫和Granzyme B的进化作用提供了新的视角.
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