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在行动中可视化病毒RNA包装信号

Emma Wroblewski¹, Nikesh Patel¹, Abid Javed¹

¹Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.

Journal of molecular biology

|August 30, 2024

概括

卫星烟草死亡病毒-1

科学领域:

病毒学病毒学
结构生物学结构生物学
分子生物学分子生物学

背景情况:

卫星烟草死亡病毒-1 (STNV-1) 形成T=1类病毒粒子 (VLPs).
病毒中的基因组包装对于感染和组装至关重要.

研究的目的:

研究包装信号 (PSs) 在STNV-1基因组包装中的作用.
阐明病毒样粒子 (VLP) 组装和基因组挤出机制.

主要方法:

组装竞争试验中的基因组规模RNA片段.
不对称的冷电子显微镜 (cryo-EM) 重建.
对RNA-蛋白相互作用和VLP结构的分析.

主要成果:

在STNV-1基因组上的多个包装信号 (PSs) 与外衣蛋白 (CPs) 进行序列特定接触,核化VLP形成.
CP N-终端残留物与gRNA PS循环序列相互作用,解释了选择性基因组包装.
改变的PS-CP亲和力导致VLP内不对称的PS分布,影响基因组挤出.
基因组折叠和CP afinities决定了病毒组合和感染中的角色.

结论:

不同的包装信号折叠和外套蛋白亲和性对于组装和感染中的病毒基因组功能至关重要.
这些发现揭示了一种由RNA折叠和VLP结构调节的基因组挤出的新机制.
保存的外套蛋白折叠表明这些基因组功能在许多病毒中普遍存在.

关键词:

基因组调节病毒组合和感染分子挫折感分子挫折感包装信号包装信号病毒组装组合的病毒组合.

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