基于单克隆抗体的合金免疫染色学试验,用于视觉和快速查profenofos
Ri-Sheng Chen1, Jian-Cheng Yuan1, Chong-Lan Luo2
1Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
Environmental pollution (Barking, Essex : 1987)
|August 30, 2024
概括
一种新的单克隆抗体 (mAb) 能够进行快速的合金免疫染色学测定 (GICA) 来检测 (PFF) 杀虫剂残留物. 该工具可快速可靠地对食品和环境样本进行查.
科学领域:
- 农业化学 农业化学
- 免疫技术是一种免疫技术.
- 分析化学 分析化学
背景情况:
- 普罗芬 (PFF) 是一种有机类杀虫剂,具有潜在的环境和健康风险.
- 由于这些风险,在土壤,水和作物中监测PFF残留物至关重要.
- 现有的检测方法可能耗时,需要专门的设备.
研究的目的:
- 为了开发一种新型单克隆抗体 (mAb),特异于profenofos (PFF).
- 建立基于mAb的快速和灵敏的合金免疫染色学测定 (GICA) 以检测PFF.
- 通过分子模拟来研究PFF识别机制.
主要方法:
- 制备和描述一种新的抗PFF单克隆抗体 (mAb).
- 分子模拟以调查抗体-抗原识别机制.
- 开发和优化基于mAb的合金免疫染色学试验 (GICA).
- 使用间接竞争性免疫吸收酶相关的免疫吸收剂测定 (ic-ELISA) 和气体染色学-双重质谱法 (GC-MS/MS) 的验证.
主要成果:
- 在ic-ELISA中,mAb显示了PFF的高特异性,IC50为12.9 ng/mL,LOD为4.6 ng/mL.
- 优化的GICA实现了20 ng/mL的视觉LOD (vLOD) 和5.2 ng/mL的定量LOD (qLOD).
- 该GICA显示线性检测范围从10.0到83.8 ng/mL.
- 在矩阵和恢复测试中,GICA结果和GC-MS/MS之间发现了良好的相关性.
结论:
- 一种新型的mAb和一个敏感的GICA已经成功地开发出用于检测profenofos.
- 该GICA提供快速 (在15分钟内) 和可靠的PFF在食品和环境样本中的选.
- 这种测量方法是监测杀虫剂残留物和确保食品安全的宝贵工具.
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