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巨型病毒作为抗生素耐药性基因的储存库
Xinzhu Yi1, Jie-Liang Liang1, Ping Wen1
1Institute of Ecological Science, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou, PR China.
Nature communications
|August 30, 2024
概括
核细胞质大DNA病毒 (NCLDV) 携带抗生素耐药性基因 (ARG),可能在生态系统中传播它们. 这些巨型病毒显示了ARG,移动元素和毒性因子之间的显著相关性.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 微生物学 微生物学
背景情况:
- 核细胞质大DNA病毒 (NCLDV),也称为巨型病毒,感染多种类型的真核生物并与各种生物进行基因交换.
- 以前的研究表明,一些NCLDV具有抗生素耐药性的基因,这表明它们在传播抗生素耐药性基因 (ARG) 中发挥了作用.
- 在NCLDV族群中,ARG的流行,演变,传播和与毒性因子的联系在很大程度上未被调查.
研究的目的:
- 在NCLDV基因组的大数据集中系统分析ARG的存在和特征.
- 探索NCLDV编码的ARG的进化起源和传播潜力.
- 调查ARG与NCLDVs中的移动遗传元素或毒性因子之间的关联.
主要方法:
- 对1416个NCLDV基因组进行全基因组调查,包括培养分离物和元基因组组装基因组.
- 对NCLDV编码的ARG进行遗传学分析,以推断其获取途径.
- 在大肠杆菌 (Escherichia coli) 中对三甲胺耐药基因的功能验证.
- 在ARG,移动遗传元素和毒性因子之间进行统计相关性分析.
主要成果:
- 在分析的NCLDV基因组中,39.5%含有ARG,与菌体基因组相比,患病率明显高.
- 在NCLDV族群中发现了12种不同的ARG类型.
- 遗传学分析表明,NCLDV从真核生物, prokaryotic 和菌体来源获得ARG.
- 证实NCLDV编码的三甲抗性基因在大肠杆菌中产生抗性.
- 在NCLDV基因组中观察到ARG和移动遗传元素和毒性因子存在的显著正相关性.
结论:
- NCLDVs是ARG的重要储库和潜在载体,有助于它们在环境中传播.
- 通过NCLDV获得ARG是多方面的,涉及从不同来源的水平基因转移.
- 在NCLDV中,ARG与移动遗传元素和毒性因子的同时出现突显了对公共健康和生态系统动态的潜在风险.
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