时间序列污水元基因组学区分季节性,人源和环境微生物群落,可能允许源归因监测
Ágnes Becsei1, Alessandro Fuschi2, Saria Otani3
1Department of Physics of Complex Systems, ELTE Eötvös Loránd University, Budapest, Hungary.
Nature communications
|August 30, 2024
概括
污水元基因组学可以识别新型微生物并追踪城市人口中的抗菌素耐药性 (AMR). 恢复新的基因组可以提高监测的准确性和对微生物动态的理解.
科学领域:
- 环境微生物学环境微生物学
- 基因组学就是基因组学.
- 公共卫生监督是对公共卫生的监督.
背景情况:
- 污水元基因组学对于对病原体和抗菌素耐药性 (AMR) 的城市监测至关重要.
- 基于参考的方法面临数据库偏差,原因是污水样本中的未知物种.
- 需要新的基因组恢复,以提高人口级微生物监测的准确性.
研究的目的:
- 从城市废水中恢复新的污水衍生的基因组.
- 开发一个工作流程来量化和关联这些基因组与AMR随时间推移.
- 评估微生物社区动态对监测工作的影响.
主要方法:
- 在五个主要欧洲城市进行纵向污水采样.
- 转基因组组装用于重建转基因组组装基因组 (MAGs).
- 网络社区分析,以探索微生物社区结构和潜在的来源归因.
主要成果:
- 检测到2332种 prokaryotic MAG,其中1334种以前没有被描述.
- 恢复的基因组约占测序DNA的69%,为微生物动力学提供了洞察力.
- 观察到季节性变化和 Pseudomonas 繁殖,这对一致的监测构成了挑战.
- 类似于人类肠道微生物群的细菌形成了独特的社区,使得新的物种和AMR基因的潜在来源归因成为可能.
结论:
- 恢复新的污水基因组显著改善了对微生物动态和AMR的理解.
- 网络社区分析显示,将新型物种和AMR基因归因于特定来源具有前景.
- 这种方法可以加强城市环境中抗菌素耐药性的跟踪.
更多相关视频
10:43Unraveling the Unseen Players in the Ocean - A Field Guide to Water Chemistry and Marine Microbiology
Published on: November 5, 2014
25.7K
12:14A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
9.8K
相关概念视频
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Methods to Assess Microbial Communities
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
Microbial Wastewater Treatment
Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Microbial Biosensors
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
Introduction to the Human Microbiota
Microorganisms colonize various regions of the human body, including the mouth, nasal passages, throat, stomach, intestines, urogenital tract, and skin. The total number of microbial cells is estimated to range from 10¹³ to 10¹⁴—comparable to, or exceeding, the number of human somatic cells. This host–microbiome relationship has led to the conceptualization of humans as supraorganisms, wherein microbial communities perform vital roles in development, immunity, and disease...
Human Virome
The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...
