相关实验视频

Updated: Jun 14, 2025

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation
08:24

CRISPR-Cas9 Genome Editing of Rat Embryos using Adeno-Associated Virus AAV and 2-Cell Embryo Electroporation

Published on: March 15, 2024

1.8K

通过AAV载体在小鼠植入前胚胎中的多级别等位基因转换

Petr Nickl1,2, Irena Jenickova3, Jan Elias3,4

  • 1Czech Centre for Phenogenomics, Institute of Molecular Genetics of the Czech Academy of Sciences, Prumyslova 595, 252 50, Vestec, Czech Republic. petr.nickl@img.cas.cz.

Scientific reports
|August 30, 2024
PubMed
概括

这项研究引入了一种有效的基于AAV的方法,用于使用Cre和FLP等特定位点重组酶 (SSR) 进行等位基转换. 这种技术简化了复杂的基因修改,减少了动物的使用,加速了基因组工程.

关键词:
3R 3R 3R 3R 3R 3R 3R 3R 3R 3R在 AAV AAV AAV 中.在Flp/FRTT上.基因输送 基因输送 基因输送在这个过程中,IVF IVF.网站特定的复合酶.

更多相关视频

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain09:59

AAV Deployment of Enhancer-Based Expression Constructs In Vivo in Mouse Brain

Published on: March 31, 2022

2.6K
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

8.8K

相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
13.9K