酸的利用极度热酸性古生物物种的酸利用
Sara Tejedor-Sanz1,2, Young Eun Song1,2, Eric R Sundstrom1,2
1Lawrence Berkeley National Laboratory, Berkeley, California, USA.
Microbial biotechnology
|August 31, 2024
概括
热酸的古生物可以利用酸,一种可持续的C1基质,用于生长. 一种新的生物反应器方法增强了酸的共同利用,导致生物技术应用中细胞密度更高.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 环境科学 环境科学
背景情况:
- 酸是一种可从可再生能源中提取的C1基质,与可持续生物制造相关.
- 格式变异通常在中性恋中进行研究;酸的毒性限制了其在酸性环境中的使用.
- 热酸具有生物技术潜力,但由于毒性,酸的利用尚未得到充分探索.
研究的目的:
- 研究来自Sulfolobales序列的热酸性古生物中的酸耐受性和代谢.
- 评估这些生物体在使用酸的生物制造应用中的潜力.
主要方法:
- 使用酸进行*Metallosphaera prunae*,*Sulfolobus metallicus*和*Sulfolobus acidocaldarium*的批量种植.
- 与酸和生理电子捐赠者 (例如铁铁) 进行共同利用实验.
- 在生物反应器中为*M. prunae*开发了一种连续的甲酸养策略.
主要成果:
- *M. prunae*, *S. metallicus* 和 *S. acidocaldarium* 用1-2毫米的酸代谢和生长.
- 酸与电子捐赠者 (如铁) 共同使用.
- 在生物反应器中的顺序养使M. prunae*能够利用16.3mM的酸,实现比单独使用H2更高的细胞密度.
结论:
- 热酸的古生物可以耐受和代谢酸,扩大已知的形式类生物的范围.
- 一个连续养生物反应器的策略增强了酸利用率和热酸友的细胞产量.
- 这项研究强调了在极端环境中酸代谢对可持续生物制造和修复的潜力.
更多相关视频
07:56Author Spotlight: Unraveling the Mysteries of Terrestrial Anaerobic Microorganisms in Uncharted Environments by In Situ Culturing
Published on: January 12, 2024
867
04:32Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Published on: October 14, 2021
2.7K
相关概念视频
01:20Fates of Pyruvate
8.4K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
8.4K
01:01Acid Halides to Carboxylic Acids: Hydrolysis
2.6K
Hydrolysis of acid halides is a nucleophilic acyl substitution reaction in which acid halides react with water to give carboxylic acids. The reaction occurs readily and does not require acid or a base catalyst.
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic...
As shown below, the mechanism involves a nucleophilic attack by water at the carbonyl carbon to form a tetrahedral intermediate. This is followed by the reformation of the carbon–oxygen π bond along with the departure of a halide ion. A final proton transfer step yields carboxylic...
2.6K