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相关概念视频

Protein Glycosylation01:25

Protein Glycosylation

6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
6.8K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

3.8K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
3.8K
Membrane Carbohydrates01:30

Membrane Carbohydrates

5.3K
The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...
5.3K
Proteoglycans01:05

Proteoglycans

3.9K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
3.9K
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

3.7K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.7K

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相关实验视频

Updated: Jun 14, 2025

Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan

Published on: September 7, 2019

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通过生物化学途径解读细胞表面的糖涂层.

Pritam Ghosh1

  • 1Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany.

Chemistry (Weinheim an der Bergstrasse, Germany)
|August 31, 2024
PubMed
概括

细胞表面上的甘氨酸对生物功能和疾病生物标志物至关重要. 新的代谢甘氨酸标记和酸探针为研究这些细胞表面糖在现场提供了先进的方法.

科学领域:

  • 碳水化合物化学 碳水化合物化学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 细胞表面的葡萄糖,形成葡萄糖,对于细胞结构,通信和治疗向至关重要.
  • 甘氨酸是复杂的,以甘油脂,甘氨酸蛋白和蛋白质甘氨酸的形式存在,这给研究带来了挑战.
  • 了解细胞甘化对于基础研究和开发针对甘的治疗方法至关重要.

研究的目的:

  • 审查甘氨酸作为疾病生物标志物的重要性.
  • 讨论在它们的生理环境中评估glycans in situ的必要性.
  • 探索先进的甘氨酸分析技术的前景和潜在应用.

主要方法:

  • 代谢甘氨酸标签通过自然代谢途径引入非自然的修改到细胞甘氨酸.
  • 由贝尔托齐集团开创的代谢性寡糖体工程利用了糖救援途径.
  • 基于酸的探针可以在没有表面修改的情况下进行单步碳水化合物识别.

主要成果:

  • 代谢性甘氨酸标签允许探针集成到细胞甘氨酸结构中,通过点击化学实现检测.
  • 酸探针提供了一种直接方法,用于在现场检测碳水化合物.
  • 该审查强调了甘氨酸作为生物标志物的重要性以及需要进行现场评估.
关键词:
这就是AI-ML.铜点击化学的化学成分葡萄糖甘是什么? 葡萄糖甘是什么金属合金的金属化物.目标治疗方法的目标疗法.

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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells

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结论:

  • 先进的甘氨酸分析技术,如代谢标记和酸探针,对于基础研究和治疗开发至关重要.
  • 甘氨酸具有作为各种疾病的生物标志物的巨大潜力.
  • 在他们的生理背景下对甘氨酸的现场评估对于理解它们的作用和应用至关重要.