一个动态的WUSCHEL/Layer 1相互作用指导烟草再生过程中射击角髓形成
Manoj Kumar1, Dana Ayzenshtat1, Gulzar A Rather1
1Department of Ornamental Plants and Agricultural Biotechnology, The Institute of Plant Sciences, The Volcani Center, ARO, Rishon LeTsiyon, Israel.
The Plant journal : for cell and molecular biology
|August 31, 2024
概括
这项研究揭示了烟草中 de novo shoot 角膜 (SAM) 形成是如何发生的. 它强调了WUSCHEL (WUS) 和L1层在再生过程中建立SAM静止的关键相互作用.
科学领域:
- 植物发育生物学植物发育生物学
- 再生生物学 再生生物学
- 分子遗传学 分子遗传学
背景情况:
- De novo shoot apical meristem (SAM) 器官生成对于植物再生至关重要,但早期细胞命运机制尚未完全理解.
- 在体外SAM形成与胚胎发生不同,缺乏位置线索,依赖于自主模式.
研究的目的:
- 调查烟草组织培养中SAM器官生成的初始阶段.
- 阐明调节SAM恒温的基因模式的分子机制.
- 在SAM形成过程中,描述WUS表达和L1层之间的相互作用.
主要方法:
- 在烟草中使用基因沉默技术 (例如,WUS和TONNEAU1沉默)
- 显微镜观察突起的形成和细胞模式.
- 对基因表达模式 (WUS) 和细胞分裂模式的分析.
主要成果:
- SAM 器官生成始于突起的形成和L1 层的建立.
- 确定了WUS表达和L1层完整性之间的复杂相互依赖.
- 沉默WUS干扰了L1形成和外细胞层组织.
- TONNEAU1沉默阻碍了L1细胞身份的获取和SAM的形成.
结论:
- 在SAM器官生成过程中,WUS在建立和组织L1层方面发挥着至关重要的作用.
- TONNEAU1对于L1细胞身份和随后的SAM形成至关重要.
- 为SAM再生中的WUS/L1相互作用提出了一个新的分子框架.
关键词:
孤独的家伙4 (LOG4)海洋系统层1 (ML1)多 (TON1) 是一个多的货币.乌舍尔 (WUS) 的意思表皮细胞分化的表皮细胞分化介质系统的形成.形态发生 (morphogenesis) 是一种形态的产生.射击顶端的美里系统.更多相关视频
相关概念视频
03:02Primary and Secondary Growth in Roots and Shoots
57.0K
Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
57.0K
02:36Meristems and Plant Growth
45.7K
Plants grow throughout their lives; this is called indeterminate growth, and it distinguishes plants from most animals. Although certain parts of plants stop growing (e.g., leaves and flowers), others grow continuously—like roots and stems.
45.7K
02:19Morphogenesis
27.8K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
27.8K
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
01:33Whole Body Regeneration
3.3K
Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential;...
3.3K
01:41Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K