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对中性粒细胞相关的枢纽基因和在败血性心肌病中ceRNA网络构建的生物信息学分析

Qingfei Cao¹, Jing Li², Meixue Chen²

¹Department of Urology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, China.

|August 31, 2024

概括

败血性心肌病 (SCM) 在败血症期间涉及心脏功能障碍. 这项研究确定MRC1是参与SCM的关键中性粒细胞相关基因,为早期诊断和治疗提供了潜在的生物标志物.

科学领域:

心脏病学心脏病学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

败血性心肌病 (SCM) 是一种严重的败血症并发症,导致心脏抑郁.
中性粒细胞在败血症引起的器官损伤中发挥作用,但它们在SCM中的特定功能尚不清楚.
鉴定中性粒细胞相关基因 (NRG) 对于理解SCM和发现诊断/治疗生物标志物至关重要.

研究的目的:

调查中性粒细胞相关基因 (NRG) 在败血性心肌病 (SCM) 病变发生过程中的作用.
根据NRG,识别基于SCM的潜在诊断和治疗生物标志物.
阐明涉及中性粒细胞在SCM中的分子机制.

主要方法:

对GSE79962和GSE44363数据集的差异基因表达分析,以确定共享的DEG.
使用Cytoscape软件和cytoHubba插件精确定位枢纽DEG.
集成的枢纽DEG与WGCNA的NRG,以确定与中性粒细胞相关的枢纽基因 (NRHG) MRC1.
在SCM中使用内部和外部数据集验证MRC1表达式.
构建并验证了一个与中性粒细胞相关的竞争性内源性RNA (ceRNA) 网络 (AC145207.5/miR-23a-3p/MRC1).

主要成果:

在败血症和对照组之间确定了共享的差异表达基因 (DEG).
确定了MRC1作为关键的中性粒细胞相关枢纽基因 (NRHG),涉及到SCM.
通过验证研究证实SCM患者MRC1的异常表达.
建立了一个功能性中性粒细胞相关的ceRNA网络,涉及AC145207.5,miR-23a-3p和MRC1.

结论:

MRC1被确定为一个潜在的NRHG,有助于SCM病原体.
这项研究提供了对中性粒细胞介导的SCM背后机制的见解.
MRC1成为SCM早期诊断和治疗干预的有希望的分子标.

关键词:

在MRC1中,MRC1是MRC1.生物标志物生物标志物网络 ceRNA 网络与中性粒细胞相关的基因败血性心肌病症是什么意思

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