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对18型,45型和59型人类乳头瘤病毒的三重类型仿制疫苗的表征

Xin Chi¹, Feng Han¹, Yanan Jiang¹

¹State Key Laboratory of Vaccines for Infectious Diseases，Xiang An Biomedicine Laboratory，School of Public Health, School of Life Sciences, Xiamen University, Xiamen, 361102, China; State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics，National Institute of Diagnostics and Vaccine Development in Infectious Diseases，National Innovation Platform for Industry-Education Integration in Vaccine Research, Xiamen University, Xiamen 361102, China.

|August 31, 2024

概括

这项研究开发了一种针对人类乳头瘤病毒 (HPV) 18,45和59型的新型仿真病毒样粒子 (VLP) 疫苗,解决了当前HPV疫苗接种中的差距. H18-45BCEF-59HI VLP候选体显示出免疫性和交叉中和性,为HPV59预防提供了潜在的解决方案.

科学领域:

病毒学和免疫学病毒学和免疫学
疫苗开发疫苗开发
分子工程分子工程分子工程

背景情况:

持续高风险的人类乳头瘤病毒 (HPV) 感染是各种癌症的重要原因.
目前的HPV疫苗并不涵盖所有高风险类型,为HPV59.9等类型留下了一个空白.
开发更广泛的HPV疫苗对于全面预防癌症至关重要.

研究的目的:

设计和开发一种针对HPV型18,45和59的仿真病毒样粒子 (VLP) 疫苗.
创建一个候选疫苗,以解决对HPV59的未满足需求,一种目前商业疫苗未涵盖的类型.
为设计针对多种病毒亚型的疫苗提供基础方法.

主要方法:

通过在HPV18,HPV45,和HPV59之间交换表面循环来工程化模拟VLP.
使用传输电子显微镜,HPSEC,AUC和DSC进行了候选构造 (H18-45BCEF-59HI) 的表征.
评估了与仿真VLP免疫接种后小鼠的免疫性和交叉中和.

主要成果:

H18-45BCEF-59HI仿真VLP的颗粒大小,形态和热稳定性与野生型VLP相似.
免疫研究显示,与H18-45EF-59HI仿真VLP的最佳交叉中和.
在类似于GMP的条件下生产的H18-45BCEF-59HI VLP被确定为一个有前途的疫苗候选者.

结论:

开发的仿真VLP是预防HPV18,HPV45和HPV59感染的潜在疫苗候选人.
这项研究提供了一个可行的策略,以解决目前高风险HPV类型的疫苗接种缺口.
该方法为开发针对各种病毒亚型的疫苗提供了一个框架.

关键词:

奇默病毒类似的粒子.人类乳头瘤病毒人类乳头瘤病毒免疫性免疫性免疫性三重类型的疫苗疫苗.

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