飞行时间木材识别数据库的实验室间和跨平台可访问性
Cady A Lancaster1, G Asher Newsome2, Julia Campbell-Such3
1Royal Botanic Gardens, Kew, United Kingdom.
Forensic science international
|August 31, 2024
概括
一项新的研究表明,Orbitrap质谱仪可以准确地识别树种,与已建立的飞行时间质谱仪 (TOF-MS) 平台的性能相匹配. 这扩大了美国鱼类和野生动物服务的可用性.
科学领域:
- * 植物学 植物学
- * 分析化学 分析化学
- * 法医科学 法医科学
背景情况:
- * 美国鱼类和野生动物管理局维护着大量的树种谱数据库,这对于打击非法砍伐和国际贸易至关重要.
- *本数据库主要是使用特定的环境电离飞行时间质谱仪 (TOF-MS) 平台构建和查询的.
- * 具有不同质谱仪器 (MS) 的实验室寻求与此数据库兼容.
研究的目的:
- * 通过使用共享数据库,将Orbitrap MS仪器与已建立的TOF-MS平台用于树种识别的有效性进行比较.
- * 评估实验室使用多种MS仪器仪表更广泛采用树种数据库的可能性.
主要方法:
- * 选择了四种树种进行实验室间比较.
- *木材样本使用Orbitrap MS和TOF-MS平台进行分析,实时电离直接分析.
- *样品的身份通过显微镜木头解剖学得到证实.
主要成果:
- * Orbitrap MS成功识别了所有测试物种,其可靠性与TOF-MS相美.
- * 轨道探测分析经常显示光谱变化比TOF-MS少.
- *在Orbitrap分析中,需要两个扫描的质量范围来实现足够的峰值强度,以实现数据库匹配.
- *与数据库匹配的质量精度和信号丰度与Orbitrap MS并非始终优越.
结论:
- * Orbitrap MS仪器是使用现有的质谱数据库识别树种的可行替代方案.
- * 该研究表明,该数据库在不同成员国平台上有更广泛的应用潜力.
- *对Orbitrap分析协议的轻微调整可以确保成功使用数据库.
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