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乳腺癌中的副信号:洞察瘤内皮细胞表型
Atarah Rass1, Carla Eksteen1, Anna-Mart Engelbrecht2
1Department of Physiological Sciences, Stellenbosch University, 2nd floor, Mike De Vries Building, Cnr. Merriman Ave & Bosman Street, Stellenbosch, South Africa.
Acta histochemica
|August 31, 2024
概括
乳腺癌细胞与正常细胞不同,显著改变内皮细胞的行为,促进血管生成. 这项研究揭示了影响瘤生长和血管化的明显的膜信号效应.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物化学 生化学
背景情况:
- 瘤内皮细胞 (TECs) 具有独特的遗传和表型特征,有助于瘤的进展.
- 来自乳腺上皮细胞的近信号影响内皮细胞的功能.
研究的目的:
- 研究来自非瘤原性 (MCF-12A) 和瘤原性 (MCF-7,MDA-MB-231) 乳腺细胞的条件介质 (CM) 对人类静脉内皮细胞 (HUVEC) 的影响.
- 分析在暴露于不同乳腺细胞CM的HUVEC中差异化的血管性反应和TEC标志物表达.
主要方法:
- 用来自MCF-12A,MCF-7和MDA-MB-231细胞系的CM对HUVEC进行治疗.
- 评估细胞活力,MCM2表达和形态变化.
- 对于TEC标记物 (MDR1,LOX,TEM8) 的定量PCR (qPCR) 和西部斑分析.
- 划痕试验用于评估HUVEC迁徙活动.
主要成果:
- 所有乳腺CM都增加了HUVEC活力,细胞类型之间存在显著差异.
- 瘤性CM诱导了更明显的HUVEC形态变化 (延长,光线形成,分支).
- 在用MCF-12A CM.治疗的HUVEC中观察到TEC标记物 (MDR1,LOX,TEM8) 的表达增加.
- 与MCF-12A CM相比,MCF-7 CM显著增强了HUVEC迁移.
结论:
- 非瘤原性和瘤原性乳腺细胞在内皮细胞中引起明显的血管性反应.
- 瘤原性乳腺癌细胞诱导了一个过度活化的血管原性表型.
- 乳腺癌细胞的膜信号差异性影响内皮细胞,突显了在血管生成中研究TEC标志物的必要性.
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