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微生物与牛奶代谢产品在冷原奶质量变化期间的关系:一个元基因组和代谢组的探索
Jiao Zhuang1, Yanru Hou1, Yuanyuan Wang1
1School of Food Science & Engineering, Ningxia University, Yinchuan 750021, China.
International journal of food microbiology
|August 31, 2024
概括
尽管冷藏,但生牛奶质量在冷藏后3-4天内就会下降. 在此期间的微生物转移和脂质代谢物变化表明质量控制的关键窗口.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
背景情况:
- 在4°C的冷藏可以延长生牛奶的保质期,但不能防止腐烂.
- 了解制冷过程中的微生物和代谢变化对于质量评估至关重要.
研究的目的:
- 分析生奶在冷藏6天内发生的物理化学和微生物变化.
- 在冷藏下探索微生物形状和牛奶代谢物之间的关系.
- 确定冷藏期间生牛奶质量变化的关键阶段.
主要方法:
- 使用Illumina Hiseq Xten测序进行元基因组学分析.
- 非定位超高性能液态染色学-四极飞行时间质谱学 (UHPLC-Q-TOF MS) 用于代谢物分析.
- 相关性分析以链接微生物和代谢数据.
主要成果:
- 主导的细菌系从Acinetobacter,Streptococcus,Staphylococcus和Anaplasma转移到Flavobacterium,Pseudomonas和Lactococcus,这些细菌系中的细菌是主要的.
- 确定了77种显著不同的代谢物,脂质是最丰富的 (37).
- 主要的代谢变化和快速增加的心理变质细菌发生在存储的3-4天之间,脂质代谢产物与Acinetobacter,Flavobacterium和Pseudomonas有很强的关联.
结论:
- 3-4天的冷藏期是原牛奶质量恶化的关键阶段.
- 针对这个3-4天的窗口可以帮助制定有效的生牛奶质量控制策略.
- 微生物群落动态和脂质代谢是冷藏期间生牛奶腐烂的关键指标.
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