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智能手机辅助的色度生物传感器用于快速视觉检测食品样本中的天然抗氧化剂

Jun Tang¹, Zhenyu Gao¹, Longfei Xu¹

¹Hunan Province Key Laboratory of Materials Surface and Interface Science and Technology, College of Materials Science and Engineering, Central South University of Forestry and Technology, Shaoshan South Road, No. 498, Changsha 410004, China.

|August 31, 2024

概括

这项研究引入了一种使用MIL-53的新方法,用于检测表甲基酸盐 (EGCG) 和氨酸 (Cys). 开发的色度传感器为这些重要的健康化合物提供了经济高效的实时分析.

科学领域:

材料科学材料科学材料科学
分析化学分析化学
生物化学生物化学

背景情况:

对表甲基酸盐 (EGCG) 和氨酸 (Cys) 的定量监测对人类健康至关重要.
开发这些化合物的高效和可访问的检测方法仍然是一个活跃的研究领域.

研究的目的:

开发一种新的色度测量传感平台,用于同时定量检测EGCG和Cys.
为了利用一种新合成的铁/双金属MOF材料,MIL-53 (Fe,Al) 的过氧化酶类 (POD类) 活性.

主要方法:

在室温下通过共同沉快速合成MIL-53 ((Fe,Al) 双金属MOF材料.
使用3,3',5,5'-四甲丁 (TMB) 作为染色基质,对MIL-53 ((Fe,Al)) 的POD类活性进行研究.
开发基于智能手机的色度测量传感平台,用于EGCG和Cys检测.

主要成果:

对于TMB和H2O2基质的迈凯利斯-门常数分别确定为0.167mM和0.108mM.
传感平台对EGCG (1580μM,R2=0.994) 和Cys (795μM,R2=0.998) 显示出极好的线性.
实现了低检测极限:EGCG为0.719微米,Cys为0.363微米.

结论:

MIL-53 ((Fe,Al) 具有显著的POD类活性,可进行敏感的色度检测.
开发的基于智能手机的平台提供了一种具有成本效益和快速的方法,用于实时分析EGCG和Cys.
这种方法为在各种应用中监测 katechins 和氨基酸提供了一个有前途的工具.

关键词:

双金属MOFs可以使用.颜色测量方法的颜色测量方法.半氨酸 (cysteine) 是一种氨酸.甲基甲基酸 (EpiGallocatechin Gallate) 是一种甲基甲基酸.纳米酶纳米酶是一种纳米酶.

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