基于三明治酶结合的阿胺基检测法用于检测Trichomonas vaginalis
Christine Aubrey C Justo1, Miriam Jauset-Rubio2, Marketa Svobodova2
1Interfibio Consolidated Research Group, Department of Chemical Engineering, Universitat Rovira i Virgili, Tarragona, Spain(1); Pathogen-Host-Environment Interactions Research Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.
Analytical biochemistry
|August 31, 2024
概括
一种新的诊断测定Trichomoniasis (TV) 显示了快速,经济有效的查的希望. 这种与酶相关的阿巴酶测定 (ELAA) 可以准确地检测临床样本中的TV,有助于预防相关的健康并发症.
科学领域:
- 医学诊断 医学诊断 医学诊断
- 生物技术是生物技术.
- 传染性疾病 传染性疾病
背景情况:
- 三虫病 (TV) 是一种流行,可治愈的非病毒性传播感染 (STI),具有重大健康影响,包括不良出生结果和不孕不育.
- 迫切需要一项强大,具有成本效益,敏感,特定和快速的电视诊断测试,特别是在低收入环境中.
- 据估计,电视在全球影响了1.56亿人,非洲和美洲的女性患病率更高.
研究的目的:
- 为检测Trichomonas vaginalis (TV) 进行敏感的三明治酶相关的胺酶测定 (ELAA) 选和优化胺酶.
- 为了评估在临床标本中针对电视检测的优化aptamer基试验的诊断性能.
主要方法:
- 选6个全长和4个截断的阿帕特马,用于基于微板的三明治试验.
- 优化用于电视检测的双相受体组合 (A1_14mer捕获和A6记者相受体).
- 使用临床标本 (宫阴道洗和阴道拭子) 对湿装显微镜进行开发的ELAA的验证.
主要成果:
- 最佳的阿帕特胺对 (A1_14mer-A6) 实现了敏感的三明治酶相关的阿帕特胺测定 (ELAA),检测极限为3.02 × 10^4 TV细胞/mL.
- 在临床样本中,A1_14mer-A6 ELAA与用于电视检测的湿装显微镜有很好的相关性.
- 该试验显示了对电视节目进行经济高效的人口查的潜力.
结论:
- 开发的A1_14mer-A6 ELAA是一种用于检测电视的敏感和特定方法.
- 这种以aptamer为基础的测定对成本效益高的三病查和管理具有重大潜力,从而防止相关并发症.
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