在Chlamydomonas核糖体生物发生过程中分配作用:保存因子NIP7
Raissa Ferreira Gutierrez1, Heloisa Ciol1, Angélica L Carrillo Barra1
1São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo 13560-970, PO Box 369, Brazil.
Biochimica et biophysica acta. Proteins and proteomics
|August 31, 2024
概括
克拉米多莫纳斯 Reinhardtii NIP7 蛋白 (CrNip7) 有助于 60S 核糖体子单元组合,其功能与酵母 NIP7 类似. CrNip7与UNC-p和G补丁蛋白相互作用,并与rRNA结合.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 细胞核核糖核糖体生物发生.
背景情况:
- 核糖体生物生成 (RB) 是一种基本的,在真核生物中保存的过程,用于创建功能性核糖体子单元.
- NIP7是一种已知的核糖体生物发生因子 (RBF),对于酵母和人类等模型生物体的60S前核糖体晚期成熟至关重要.
- 在各种真核生物中存在NIP7同类物,包括Chlamydomonas reinhardtii,这表明功能保留.
研究的目的:
- 在核糖体生物生成中功能性地表征克拉米多马纳斯强化NIP7蛋白 (CrNip7).
- 为了研究CrNip7.7的潜在蛋白质-蛋白质相互作用.
- 探索CrNip7与核糖体RNA (rRNA) 的相互作用.
主要方法:
- 在酵母中进行蛋白质补充试验和帕罗摩辛耐药性试验,以评估CrNIP7的功能.
- 酵母二杂交试验以确定CrNip7的交互伙伴.
- 在 silico rRNA 建模和核酸相互作用研究中分析 CrNip7-rRNA 的结合.
主要成果:
- CrNip7成功地补充了酵母中NIP7的功能,证实了其在60S核糖体子单元生物发生中的作用.
- 两种潜在的相互作用伙伴,UNC-p和G-patch蛋白,被确定为CrNip7.
- CrNip7的N端和C端域都与rRNA直接相互作用.
结论:
- CrNip7是一种功能性的核糖体生物发生因子,参与了Chlamydomonas reinhardtii中的60S子单元组合.
- CrNip7与特定的蛋白质合作伙伴相互作用并直接与rRNA结合,突出显示了真核核糖核糖体生物发生过程中的保存机制.
- 这项研究为NIP7在真核生物物种的进化保护和功能作用提供了新的见解.
相关概念视频
Ribosomal RNA Synthesis
13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K
Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
Cotranslational Protein Translocation
7.3K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
7.3K
Nonsense-mediated mRNA Decay
10.6K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
10.6K


