叶片多边形的机制 cuspidati 在基于网络药理学,分子对接和实验药理学验证的肝-阳-活跃性高血压
Wenxin Shi1, Jinhang Zhou2, Jiang He1
1School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, 430065, China; Hubei Provincial Chinese Medicine Health Food Engineering Research Center, Wuhan, 430065, China; Hubei Shizhen Laboratory, Wuhan, 430065, China.
Journal of ethnopharmacology
|August 31, 2024
概括
在4月和9月收获的Folium Polygoni Cuspidati (HZY) 有效地降低了肝-阳-高活性高血压 (GYSK) 的老鼠的血压. 这两种HZY变异都会影响新陈代谢和信号通路,其中4月HZY显示出更强的氨基酸调节.
科学领域:
- 药理学 药理学是指药理学的学科.
- 传统中国医药 传统中国医药
- 心血管研究研究心血管研究
背景情况:
- 高血压是一个严重的全球健康问题,肝-阳-多动性高血压 (GYSK) 是最常见的类型.
- Folium Polygoni Cuspidati (HZY) 传统上在中国云南省用于治疗GYSK高血压,但其活性成分和与收获时间相关的疗效尚不清楚.
研究的目的:
- 在大鼠模型中研究4月收获的HZY和9月收获的HZY对GYSK高血压的不同影响.
- 阐明作用的潜在机制,包括代谢和信号通路的参与.
主要方法:
- 建立一个GYSK高血压大鼠模型,使用石和L-NAME.
- 评估血压,GYSK症状,心脏指数和大动脉病理.
- 通过UPLC-QTOF-MS对HZY进行化学分析.
- 网络药理学用于机制预测.
- 实验验证使用血清代谢量学和西方布洛特.
主要成果:
- 无论是4月还是9月,HZY都显著改善了GYSK症状,抑制了RAAS系统,减少了氧化应激,并调节了血液脂质.
- 在UPLC-QTOF-MS中,确定了29个HZY组件. 网络药理学建议涉及脂质代谢,PI3K/Akt,MAPK和TNF信号通路.
- 西方斑点证实了PI3K/Akt的激活和p-erk,p-p38和TNF-α的抑制. 血清代谢表明,4月HZY主要影响氨基酸代谢,而9月HZY影响脂质代谢.
结论:
- 无论是4月份还是9月份,HZY都通过调节代谢乱和影响PI3K/Akt-eNOS和MAPK信号通路,在GYSK大鼠中表现出抗高血压作用.
- 与9月份的HZY相比,4月份的HZY对氨基酸代谢具有更强的调节作用,突出了基于收获时间的独特治疗机制.
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