心脏是斑马鱼血造干细胞和祖先细胞的居住组织
Dorothee Bornhorst1,2, Amulya V Hejjaji1,2, Lena Steuter1,2
1Institute of Cell Biology, Faculty of Medicine, University of Münster, Münster, 48149, Germany.
Nature communications
|August 31, 2024
概括
斑马鱼内心产生并维持血造干细胞和前代细胞 (HSPC). 这项研究揭示了内心脏作为心脏内的新造血细胞的储存库和来源.
科学领域:
- 发展生物学 发展生物学
- 血液形成 血液形成 血液形成
- 心血管生物学 心血管生物学
背景情况:
- 造血细胞的起源,特别是造血干细胞和原生细胞 (HSPCs),是发育生物学中的一个基本问题.
- 内心细胞 (EDC) 对血液形成的贡献一直是持续辩论的主题.
- 了解内心脏的作用可以揭示血细胞形成和组织维护的新机制.
研究的目的:
- 为了研究内心细胞对斑马鱼的造血系的贡献.
- 为了确定参与血液形成的特定内心脏子群.
- 阐明维持心脏内存血液细胞的机制.
主要方法:
- 单细胞RNA测序以识别具有造血潜力的内心子群.
- 高分辨率显微镜和光转换追踪以追踪细胞起源.
- 活体心脏培养和脉动心脏的纵向光板显微镜.
- 功能性测试包括阻断原始红色素形成和评估粘附因子 (Integrin α4,Vcam1).
主要成果:
- 一个特定的内心子群体表达了丰富的造血促进基因.
- 造血细胞,包括HSPCs/巨核细胞-红色素前体 (MEPs),来自内心细胞和相邻的背动脉.
- 心脏HSPC/MEP的出现和维持得到了ex vivo培养和体内成像的支持.
- 细胞维护依赖于Integrin α4和Vcam1,但并不是显著地依赖于心脏的轨道运动或剪切应力.
- 阻断红细胞形成导致心脏造血细胞的增加,这表明内心脏充当储存库.
结论:
- 内心脏作为斑马鱼中HSPCs/MEPs的居住组织.
- 内心细胞是血液细胞的新来源.
- 内心脏代表了以前未知的血造干细胞和祖细胞的利基.
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