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相关概念视频

Transcription Factors02:16

Transcription Factors

75.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

6.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
6.4K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
General Transcription Factors01:30

General Transcription Factors

5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.3K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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相关实验视频

Updated: Jun 14, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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识别转录因子与非负矩阵因子化的共同结合模式.

Ieva Rauluseviciute1, Timothée Launay1, Guido Barzaghi2,3

  • 1Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership, University of Oslo, 0318 Oslo, Norway.

Nucleic acids research
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PubMed
概括

一种名为COBIND的新方法可以自动识别DNA上的转录因子 (TF) 联合结合模式. 这种方法揭示了共享的TF动机和功能相关的,在进化过程中保存的共同结合配置.

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A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
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科学领域:

  • 基因组学就是基因组学.
  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • 与DNA结合的转录因子 (TF) 是调节基因表达的基础.
  • 了解合作性TF与DNA结合至关重要,但具有挑战性.
  • 现有的方法缺乏全面的方法来识别TF联合约束模式.

研究的目的:

  • 开发和验证一种新的计算方法,COBIND,用于自动识别TF联合绑定模式.
  • 分析跨多个物种和TF家族的TF联合结合.
  • 评估已识别的共同结合模式的功能相关性和进化保存.

主要方法:

  • COBIND利用非负矩阵因子化 (NMF) 在围绕已知的TF结合位点 (TFBSs) 的DNA区域.
  • 该方法处理一次热编码序列数据,以在特定距离检测丰富的DNA模式.
  • COBIND被应用于一个大型数据集 (5699个TFBS) 跨越7个物种的401个TFs.

主要成果:

  • COBIND成功地确定了已知和新型的TF联合结合模式,由动机相似性和蛋白质-蛋白质相互作用数据支持.
  • 在各个物种中,67%的TF在相同的结构家族中共享共同结合的动机.
  • 预测的共同结合模式表现出更高的进化保护,并通过开放的染色质和单分子足迹数据进行验证.

结论:

  • COBIND是一个有效的工具,用于发现TF联合绑定配置.
  • 在结构相关的TF中,共同约束的动机普遍存在,这表明保留了监管机制.
  • 已识别的共同结合模式具有功能意义,并且在进化过程中得到保护,为转录调节提供了洞察力.