相关实验视频

Updated: Jun 14, 2025

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

Published on: November 30, 2016

胃癌诊断中的细胞外膀miRNAs

Gleb O Skryabin¹, Anastasiya A Beliaeva², Adel D Enikeev²

¹Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation, Moscow, 115522, Russia. go.skryabin@gmail.com.

Biochemistry. Biokhimiia

|September 1, 2024

概括

细胞外囊泡微RNAs (EV miRNAs) 显示出通过液体活检检测早期胃癌 (GC) 的生物标志物. 方法的标准化对于可靠的诊断和预后至关重要.

科学领域:

在瘤学瘤学.
生物化学生化学
分子生物学分子生物学

背景情况:

胃癌 (GC) 由于晚期诊断和高死亡率,对全球健康造成重大负担.
早期检测对于改善GC患者生存率至关重要.
确定液体活检可靠的生物标志物对于早期GC诊断至关重要.

研究的目的:

审查细胞外囊泡微RNAs (EV miRNAs) 作为胃癌诊断和预后的生物标志物的潜力.
分析用于GC生物标志物的EV和EV小RNA检测的方法方面.

主要方法:

在PubMed和Google Scholar中对原始文章进行系统的文献搜索.
专注于在Web of Science和Scopus中索引的研究.
对体液选择,EV隔离,验证和EV小RNA分析方法的分析.

主要成果:

大多数研究都使用了血液衍生的细胞外囊泡 (EVs);特定于组织的局部生物流体显示出有前途.
鉴定到的GC相关的miRNAs因不同的方法而显著变化.
三种miRNAs (miR-10b,miR-21,miR-92a) 在研究中始终与GC相关.

结论:

EV miRNAs有可能成为GC的非侵入性生物标志物.
对EV隔离和miRNA分析方法的标准化对于诊断可靠性至关重要.
需要进一步的研究,以建立一个最佳的miRNA标记面板GC.

关键词:

电动汽车 (EVs) 是一种电动汽车.早期诊断早期诊断早期诊断外基因组是外基因组的组成部分.胃癌胃癌是一种胃癌.胃汁是一种胃汁.液体活检活检液体活检这是一个小RNARNA.

更多相关视频

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...