作为和双价的标,心脏肌肉素和薄丝是和双价的目标
Oksana P Gerzen1, Iulia K Potoskueva2, Alena E Tzybina2
1Institute of Immunology and Physiology, Ural Branch of the Russian Academy of Sciences, Ekaterinburg, 620078, Russia. o.p.gerzen@gmail.com.
Biochemistry. Biokhimiia
|September 1, 2024
概括
诸如和之类的重金属通过直接影响肌和细丝功能,损害了心肌细胞的功能. (Cd2+) 对肌的毒性作用比 (Pb2+) 更强.
科学领域:
- 心血管毒理学心血管毒理学
- 细胞力学 细胞力学
- 环境健康 环境健康
背景情况:
- (Pb2+) 和 (Cd2+) 是普遍存在的环境重金属,与心血管疾病有关.
- 心肌细胞易受重金属毒性影响,影响心脏功能.
研究的目的:
- 研究和对心肌细胞功能在分子水平的直接影响.
- 确定这些重金属损害心脏收缩能力的具体机制.
主要方法:
- 利用绿色染料来追踪重金属进入和心肌细胞内的分布.
- 采用了体外运动性试验来测量Pb2+和Cd2+对actin-myosin相互作用的影响.
- 评估滑动速度的变化,力生成和丝结合.
主要成果:
- 和都进入心肌细胞,并在整个细胞中分布.
- 增加Pb2+和Cd2+的度显著降低了actin-myosin的滑动速度.
- (Cd2+) 在较低度 (0.6mM) 阻碍了薄丝的滑动,而不是 (Pb2+) (1.1-1.6mM).
- 与单独的Pb2+ (1.6 mM) 相比,Cd2+在较低度 (1.1 mM) 中也抑制了actin在myosin上滑动.
- 对于肌肉蛋白头部力产生或行为丝结合的影响,没有观察到显著的差异.
结论:
- 首次证明Pb2+和Cd2+直接损害心肌细胞中的肌和细丝功能.
- 与Pb2+相比,Cd2+对肌素功能表现出更强大的毒性作用.
- 这些发现为和对心血管的毒性提供了分子洞察力.
相关概念视频
01:26Heart Failure Drugs: Inotropic Agents
545
Positive inotropic agents are commonly used as the first line of treatment for heart failure. One such agent is digoxin, derived from the genus Digitalis, which has been known for centuries but effectively utilized since 1785. However, these cardiac glycosides can have potentially toxic effects due to their mechanism of action, which involves inhibiting Na+/K+-ATPase and increasing contractility. Digoxin is absorbed orally and distributed in various tissues, including the CNS. It has a long...
545
01:25Antianginal Drugs: Calcium Channel Blockers and Ranolazine
464
Angina pectoris, a primary symptom of ischemic heart disease, requires careful pharmacological interventions. In this context, calcium channel blockers (CCBs) and ranolazine have emerged as crucial pharmacotherapeutic agents, providing deep insights into the complexities of angina management.
CCBs, a diverse class that includes dihydropyridines (nifedipine) and diphenylalkylamines (verapamil and diltiazem), exert their effect by blocking calcium channels in cardiac and smooth muscle cells. This...
CCBs, a diverse class that includes dihydropyridines (nifedipine) and diphenylalkylamines (verapamil and diltiazem), exert their effect by blocking calcium channels in cardiac and smooth muscle cells. This...
464
01:18Antihypertensive Drugs: Action of Calcium Channel Blockers
473
Calcium ions are essential to contract smooth muscle cells in blood vessels. They enter these cells through voltage-dependent calcium channels, specifically L-type calcium channels in the cell membrane. These L-type calcium channels are integral to the excitation-contraction coupling process in smooth muscle. When a stimulus is received by smooth muscle cells, their membrane depolarizes. This alteration in membrane potential instigates the opening of L-type calcium channels. As a result,...
473
01:16Calmodulin-dependent Signaling
5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
01:27Specialized Characteristics of Cardiac Muscles
2.4K
The primary role of cardiac muscles is to propel blood throughout the cardiovascular system. The cardiac muscle cells, or cardiomyocytes, exhibit specialized characteristics that allow them to perform this function.
Cardiac muscle cells are smaller than skeletal muscles, averaging 10–20 mm in diameter and 50–100 mm in length. However, they have large energy demands for continuous contraction and relaxation. This energy is almost exclusively derived from aerobic metabolism of energy...
Cardiac muscle cells are smaller than skeletal muscles, averaging 10–20 mm in diameter and 50–100 mm in length. However, they have large energy demands for continuous contraction and relaxation. This energy is almost exclusively derived from aerobic metabolism of energy...
2.4K
01:25Smooth Muscle Contraction
2.6K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
2.6K