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来自Enterococcus faecalis的膜囊泡在中性和性pH下具有抗炎作用

Chenguang Niu¹, Jing Huang¹, Lifan Wei²

¹Department of Endodontics and Operative Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Disease, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.

Journal of endodontics

|September 1, 2024

概括

菌面膜囊泡 (MVs) 促进炎症. 来自性条件的MVs在体外和体内表现出更强的炎症效应.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
细胞生物学细胞生物学

背景情况:

菌面膜囊泡 (MVs) 涉及到各种生物过程.
来自不同pH条件的MVs对炎症的影响尚未完全理解.

研究的目的:

研究Enterococcus faecalis膜囊泡 (MVs) 在中性 (pH 7.4) 和性 (pH 9.0) 条件下产生的促炎作用.
描述E. faecalis MVs的蛋白质含量及其对体外和体内炎症反应的影响.

主要方法:

在此过程中,E. faecalis MVs被分离和净化.
蛋白质组分析确定了MV蛋白成分.
用MVs刺激THP-1巨细胞以评估炎症性细胞因子和蛋白质表达.
在体内研究包括小鼠血清细胞因子分析和MV注射后脏细胞免疫类型.

主要成果:

蛋白质组分析显示,中性和性条件下的MV之间蛋白质组成存在显著差异.
E. faecalis MVs诱导了依赖度的IL-1β,IL-6和TNF-α的分泌.
MV暴露增加了亲IL-1β,NF-κBp65和TLR2的表达.
在体内,MVs促进了IL-1β分泌和脏细胞激活,性衍生MVs显示了增强的效果.

结论:

Enterococcus faecalis MVs含有毒性相关的蛋白质,可以在体外和体内诱导炎症.
从性培养条件中获得的MVs表现出更强大的抗炎能力.

关键词:

菌 (Enterococcus faecalis) 是一种菌.免疫细胞免疫细胞炎症刺激刺激的炎症刺激巨细胞是什么?巨细胞是什么?膜囊泡中的膜泡.蛋白质基质的保护性

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