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从葡萄糖中高效地生产糖,并在细胞质生产中潜在的工业应用

Haiqian Yang¹, Yejun Han¹, Xiaowei Peng¹

¹National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing 100190, China; School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.

Bioresource technology

|September 1, 2024

概括

使用CoGH1A酶的高效粉生产使工业纤维素制造成为可能. 这一突破克服了以前的局限性,为增强的酶生产和应用铺平了道路.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
工业微生物学工业微生物学

背景情况:

苏是Trichoderma reesei细胞酶生产的关键诱导剂.
目前的糖生物合成方法效率低下,限制了工业细胞质生产.

研究的目的:

开发一种使用CoGH1A酶生产索的高效方法.
通过克服索的可用性限制,实现工业规模的纤维素生产.

主要方法:

利用CoGH1A,一种热友性糖化物酸酶,通过转糖酶化葡萄糖来生产索.
优化反应条件以最大限度地提高酸盐的产量和生产率.
开发可回收Fe3O4-CS-THP-CoGH1A纳米颗粒,以提高酶的可重复使用性.
在Trichoderma reesei细胞酶诱导过程中应用了产生的富含的混合物 (MGDC).

主要成果:

实现了 37.86 g/L 的最大糖产量,生产率为 9.47 g/L/h,是报告中最高的.
证明了Fe3O4-CS-THP-CoGH1A纳米颗粒的高酶稳定性,在5个循环后保持83.90%的活性.
通过使用CoGH1A衍生索酶诱导剂,达到188.38 FPU/L/h的细胞酶生产率.

结论:

CoGH1A通过转糖酶化有效地从葡萄糖中产生苏.
可回收的CoGH1A纳米颗粒促进了可持续和经济高效的粉生产.
这种方法确立了松作为一个可行的诱导剂,用于增强细胞质生产.

关键词:

蜂酶生产的生产方式固定化固定化固定化索福洛斯 (Sophorose) 是一个诗人.经过糖基化转化.三斑复合症 (Trichodema reesei) 是一个这就是β-D-葡萄糖酶.

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