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大菌向石墨烯氧化物纳米系统协同杀死抗生素和宿主免疫防御结核病治疗

Jiang Pi¹, Dongsheng Chen², Jiajun Wang²

¹Research Center of Nano Technology and Application Engineering, The First Dongguan Affiliated Hospital, Dongguan Innovation Institute, Guangdong Medical University, China; Guangdong Provincial Key Laboratory of Medical Immunology and Molecular Diagnostics, School of Medical Technology, Guangdong Medical University, Dongguan, Guangdong, China.

Pharmacological research

|September 1, 2024

概括

一种新的曼诺斯功能化的石墨烯氧化物系统为巨细胞提供抗生素和自诱导剂,增强结核病治疗. 这种有针对性的方法可以提高药物的有效性和宿主免疫力,从而更好地清除 Mycobacterium tuberculosis.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
传染性疾病传染性疾病

背景情况:

结核病 (TB) 仍然是全球主要的传染病挑战.
目前的治疗方法难以准并增强宿主免疫反应以对抗Mycobacterium tuberculosis (Mtb).
耐药结核病需要创新的治疗策略.

研究的目的:

开发一种针对巨细胞的纳米系统,用于同时提供抗结核病药物和自诱导剂.
通过将向化疗与宿主免疫调节相结合,增强细胞内杀死Mtb.
评估开发的纳米系统的有效性和安全性在体外和体内.

主要方法:

设计和合成曼诺糖功能化,聚乙胺保护的石墨烯氧化物 (GO-PEI-MAN) 纳米粒子.
在GO-PEI-MAN系统 (Rif@Car@GO-PEI-MAN) 上加载里法素 (抗生素) 和卡巴马西平 (自诱导剂).
在受感染小鼠中评估巨细胞吸收,药物释放,Mtb杀死,自促进,亡诱导,M1极化,细胞因子产生和体内疗效.

主要成果:

GO-PEI-MAN在体外和体外都显示了巨细胞的选择性吸收.
Rif@Car@GO-PEI-MAN在酸性溶酶体环境中证明了药物释放的加速.
该纳米系统显著促进了Mtb感染的巨细胞的自,亡和M1两极分化,增加了抗菌因子.
在小鼠中,Rif@Car@GO-PEI-MAN表现出优异的细胞内MTB杀伤和降低的肺部菌群负担,病理性减少,没有全身毒性.

结论:

针对巨细胞的GO-PEI-MAN纳米系统有效地联合提供抗结核病药物和自诱导剂.
这种协同方法增强了药物中介杀戮和对MTB的宿主免疫防御.
开发的纳米系统作为结核病和耐药结核病的新型治疗策略具有前景.

关键词:

药物运输药物运输药物运输石墨烯氧化物石墨烯氧化物主体免疫学清除宿主免疫学清除针对巨细胞的向结核病菌菌菌的结核病菌.

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