用磁性预度和基于探针的免疫试验合用于尿分析中的诱导合血质谱的aquaporin-1蛋白质的定量方法
Shuqi Liang1, Shuang Zhao2, Hongtao Liu3
1Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou, 510275, China; MOE Key Laboratory of Bioinorganic and Synthetic Chemistry/KLGHEI of Environment and Energy Chemistry, School of Chemistry, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510006, China.
Analytica chimica acta
|September 1, 2024
概括
一种新方法使用磁珠和ICP-MS.精确检测尿液中的Aquaporin-1 (AQP1). 这种敏感的测试通过量化AQP1蛋白水平,显示了早期疾病查的潜力.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 水素-1 (AQP1) 对于水的平衡至关重要,在脏中大量存在.
- 检测尿液AQP1可以帮助早期疾病查.
- 传统方法在复杂样本中缺乏AQP1检测的准确性和灵敏性.
研究的目的:
- 开发一种敏感和特定的方法来检测人体尿液中的Aquaporin-1 (AQP1) 蛋白.
- 使用磁性预度和基于探针的免疫试验与ICP-MS相结合,以加强检测.
主要方法:
- 使用磁珠 (MBs) 进行AQP1丰富的元素标记策略.
- 在金纳米颗粒上使用抗AQP1抗体的探针用于特定的AQP1识别.
- 感应合等离子体质谱 (ICP-MS) 用于精确的量化和信号放大.
主要成果:
- 该测试通过金原子实现了14,000倍的信号放大.
- 获得了0.023 ng/mL的低检测极限.
- 证明了卓越的特异性和广泛的线性范围 (0.330 ng/mL).
结论:
- 开发的方法成功量化了人类尿样中的AQP1蛋白.
- 这种技术具有准确的AQP1量化和早期疾病查的潜力.
- 该平台可以适应使用特定抗体选其他蛋白质.
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