高质量的FLORET UTE成像用于临床翻译
Matthew M Willmering1,2, Guruprasad Krishnamoorthy3,4, Ryan K Robison5,6
1Center for Pulmonary Imaging Research, Division of Pulmonary Medicine, Cincinnati Children's Hospital, Cincinnati, Ohio, USA.
Magnetic resonance in medicine
|September 2, 2024
概括
本研究介绍了一种改进的3D超短TE (UTE) 成像协议FLORET,为广泛的MRI应用提供高质量的临床诊断图像,显著减少扫描和重建时间.
科学领域:
- 磁共振成像技术 磁共振成像技术
- 医学成像技术 医学成像技术
- 生物医学工程 生物医学工程
背景情况:
- 开发强大的3D超短TE (UTE) 协议对于高质量的临床成像至关重要.
- 之前的费马循环直角编码轨迹 (FLORET) 是有前途的,但需要进一步优化.
- 尿道瘤序列的临床翻译需要可复制,快速和诊断准确的成像.
研究的目的:
- 开发和验证一个强大的3D UTE协议,适合临床翻译.
- 为了实现可重复的,高质量的成像与临床上可接受的扫描时间.
- 在各种应用程序和扫描仪中评估开发的UTE协议的诊断质量.
主要方法:
- 使用费马循环直角编码轨迹 (FLORET) 优化了一个UTE序列.
- 实施的修改包括梯度波形频率限制,轨迹排序,平衡的SSFP,快速梯度破坏和直线重建.
- 在不同地点的多个MRI扫描仪上获取幽灵和人类对象的图像.
主要成果:
- 增强的FLORET协议在幻影,肌肉骨和肺部应用中产生了高质量的图像.
- 通过梯度波形和轨迹顺序修改,人工物被显著减少.
- 获取和重建时间缩短 (MSK/肺部总<4分钟),在各站点的诊断图像质量一致.
结论:
- 最近的改进使得可靠,高质量的3D UTE成像,采集和重建时间短.
- 优化的FLORET序列显示为临床UTE成像准备.
- 在多个扫描仪和站点上成功实施验证了协议的稳定性,而不需要额外的系统校准.
相关概念视频
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
02:07Improving Translational Accuracy
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
02:07Improving Translational Accuracy
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
01:17Transcription
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
01:31Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are called the...
Translation Produces the Building Blocks of Life
Proteins are called the...