相关实验视频

Updated: Jun 14, 2025

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

ExomiRHub:用于托管和分析与人类疾病相关的细胞外微RNA转录学数据的全面数据库

Yang Liu^1,2,3,4, Zhuochao Min^5,6, Jing Mo⁴

¹The Key Laboratory of Advanced Interdisciplinary Studies, The First Affiliated Hospital of Guangzhou Medical University; GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Guangzhou 510182, China.

Computational and structural biotechnology journal

|September 2, 2024

概括

ExomiRHub是一个用户友好的数据库,为生物学家标准化细胞外微RNA (miRNA) 数据. 它加速生物标志物发现,正如通过识别miRNA生物标志物来确定质瘤进展所示.

科学领域:

生物医学信息学生物医学信息学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

细胞外微RNA (miRNA) 表达数据在研究中表现出显著的异质性,阻碍了缺乏生物信息学专业知识的生物学家进行分析.
标准化和精心策划的数据集对于可靠的miRNA生物标志物发现和解释至关重要.

研究的目的:

开发一个用户友好的数据库,ExomiRHub,整合生物研究人员的各种细胞外miRNA表达数据集.
为探索miRNA表达模式和识别潜在生物标志物提供全面的分析工具.

主要方法:

整合了191个人类细胞外miRNA表达数据集 (112种疾病,62种治疗方法,24种基因型) 和来自癌症基因组图谱的16012个miRNA转录组.
标准化和策划所有数据与注释.
开发了25个分析功能,包括差异表达,共同表达,权重基因共同表达网络分析 (WGCNA) 和功能丰富分析.
集成了一个用于用户数据分析的网络服务和四个额外的miRNA专注的工具.

主要成果:

ExomiRHub包含了广泛的细胞外miRNA数据,涵盖了许多疾病表型,治疗方法和基因型.
该平台提供先进的分析功能,允许定制数据探索和生物标志物识别.
成功识别了与血管生成相关的细胞外miRNA生物标志物,用于监测质瘤进展.

结论:

对于生物学家来说,ExomiRHub显著简化了复杂的细胞外miRNA表达数据的分析.
数据库及其工具有可能加速发现和验证各种疾病的细胞外miRNA生物标志物.
促进细胞外miRNA生物标志物发现的研究,有助于疾病监测和进展评估.

关键词:

癌症癌症癌症癌症异构体是什么? 异构体是什么? 外构体是什么?细胞外囊泡细胞外囊泡液体活检液体活检这就是米RNA.

更多相关视频

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice

Published on: January 7, 2019

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...