相关实验视频

Updated: Jun 14, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

基因型选择通过对小麦农学重要特征的定量特征局部映射来确定精英系

Yunlong Pang¹, Liming Wang², Linzhi Li³

¹National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai'an, China.

Molecular breeding : new strategies in plant improvement

|September 2, 2024

概括

研究人员确定了12个定量特征位点 (QTL) 基因,这些基因显著提高了小麦每种植物的理论产量 (TYP). 这种遗传洞察力有助于通过分子育种和标记器辅助选择开发高产小麦品种.

科学领域:

植物遗传学植物遗传学
农业学是一种农业学.
分子育种是一种分子育种.

背景情况:

小麦是全球重要的一种主要粮食作物.
通过基因改造提高小麦产量对于粮食安全至关重要.
了解产量相关特征的遗传基础对于分子育种策略至关重要.

研究的目的:

确定与小麦产量相关特征相关的定量特征位点 (QTL).
发现候选基因并开发分子标记物,以提高每种植物的理论产量 (TYP).
选择精英小麦生产线,使用增强的TYP.

主要方法:

从Jimai 22 (JM22) 和Cunmai no.1 (CM1) 之间的交叉中发展出一个复合杂交 (RIL) 种群.
使用7359个单核酸多态 (SNP) 标记器构建一个高密度遗传图.
在六个环境中对八种与产量相关的特征进行定量特征位置 (QTL) 映射,随后进行候选基因识别和KASP标志物开发.

主要成果:

61个QTL被确定为八个与产量相关的特征.
17个QTL预测了每个植物的理论产量 (TYP),其中12个QTL等位基因对TYP有积极的贡献.
选择了四个精英线,TYP增加了5.6%-15.2%,并对12个QTL的高通量KASP标记器进行了验证.

结论:

该研究确定了关键的QTL和候选基因,这些基因对小麦产量有显著的贡献.
开发的精英生产线和经过验证的KASP标记器为在小麦育种中使用标记器辅助的选择提供了宝贵的工具.
这些发现将加速高产小麦品种的发展.

关键词:

候选基因是一个候选基因.具有竞争力的等位基特异性PCR.链接映射链接映射标记器辅助的选择小麦的农学特征

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Published on: March 13, 2020

相关概念视频

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

Dihybrid Crosses

Dihybrid Crosses

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.