相关实验视频

Updated: Jun 14, 2025

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
12:16

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

24.2K

基于蛋白质的融合蛋白:用于可溶性生产和方便净化重组蛋白的伟大的标签

Fatemeh Shafiee1,2, Shima Sharifi1, Armin Amini3

  • 1Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

Iranian journal of biotechnology
|September 2, 2024
PubMed
概括

蛋白质标签增强可溶性重组蛋白的表达,并通过自我裂变简化大肠杆菌的净化,避免使用恶劣的化学物质或蛋白酶. 这种方法可以提高活性蛋白的产量.

关键词:
基结合域是一个基结合域.在英特因中,英特因是净化 净化 净化 净化可溶性表达的可溶性表达

更多相关视频

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach11:20

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

53.8K
Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration12:03

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration

Published on: January 22, 2014

33.6K

相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K