相关实验视频

Updated: Jun 14, 2025

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

通过激素和转录基因分析,阐明控制Angelica sinensis颜料生物合成的AsANS

Khadija Tehseen Arshad^1,2,3, Chunfan Xiang^1,2,3, Chengxiao Yuan^1,2,3

¹Key Laboratory of Medicinal Plant Biology of Yunnan Province, National & Local Joint Engineering Research Center on Germplasms Innovation & Utilization of Chinese Medicinal Materials in Southwest China, Yunnan Agricultural University, Kunming, China.

Physiologia plantarum

|September 2, 2024

概括

酸是控制Angelica sinensis.紫色的关键. 过度表达AsANS基因显著增加了安东素的产生,为培育这种药用植物提供了洞察力.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
传统中国医药传统中国医药

背景情况:

天,一种传统的中国药用植物,因其营养特性而受到重视.
植物色素,特别是紫色,是影响A. sinensis.商业价值的关键特征.
了解色素的遗传和荷尔蒙调节对于作物改善至关重要.

研究的目的:

阐明控制Angelica sinensis.紫色的分子机制.
为了确定关键的基因和激素参与安托氨酸生物合成.
探索基因操纵对增强色素的潜力.

主要方法:

在两个对比的A. sinensis品种的三个组织 (叶子,根,茎) 上进行了荷尔蒙和转录基因分析.
权重基因共同表达网络分析 (WGCNA) 用于识别基因模块和枢纽基因.
基因功能通过在转基因细胞中过度表达候选基因 (AsANS) 来验证.

主要成果:

亚甘酸被确定为一种关键的激素,调节了A. sinensis.中的安东氨酸生物合成.
鉴定和验证了七个关键基因在安托素生物合成途径.
该ANS基因被确定为一个特定WGCNA模块中的枢纽基因.
在转基因中过度表达AsANS导致了氨酸积累和紫色的增加.

结论:

这项研究揭示了在A. sinensis.中紫色的基础是动态的激素和基因表达网络.
酸和特定的安托氨酸生物合成基因,特别是AsANS,在色素中起着至关重要的作用.
这些发现为培育策略提供了基础,以提高A. sinensis.的安东素生产和商业价值.

更多相关视频

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Annotation of Plant Gene Function via Combined Genomics, Metabolomics and Informatics

Published on: June 17, 2012

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics

Published on: November 20, 2017

相关概念视频

Chromatin Modification in iPS Cells

Chromatin Modification in iPS Cells

Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...

The Antenna Complex

The Antenna Complex

Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...