非正规的双价基因Wfdc15a控制精子生成蛋白酶和免疫常态稳定
Shin-Ichi Tomizawa1, Rachel Fellows1, Michio Ono1
1Department of Histology and Cell Biology, Yokohama City University School of Medicine, Yokohama 236-0004, Japan.
概括
WFDC15A对男性生育能力至关重要,因为其缺乏会破坏精子发育,并引发炎症反应. 这项研究揭示了一种独特的表观遗传程序,调节精子生成和男性生殖健康所必需的先天免疫力.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 免疫学 免疫学 免疫学
背景情况:
- 男性不孕症与影响精子生成的遗传和表观遗传因素有关.
- 精子干细胞 (SSC) 中的表观遗传修饰剂在精子发育过程中调节基因表达.
- 预编程基因在生育中的确切作用仍然在很大程度上是未知的.
研究的目的:
- 调查WFDC家族蛋白酶抑制剂Wfdc15a在精子生成和男性生育能力中的作用.
- 为了阐明精子发育过程中Wfdc15a的表观遗传调节.
- 了解WFDC15A缺乏对丸功能和免疫力的后果.
主要方法:
- 在SSC和化过程中分析Wfdc15a表观遗传标记 (H3K4me3,H3K9me3,H3K27ac).
- 研究WFDC15A缺乏对精子生成和丸组织学的影响.
- 评估WFDC15A耗尽对丸蛋白酶-抗蛋白酶网络和炎症标记物 (TNFα) 的影响.
主要成果:
- Wfdc15a是SSC中非正规的双价基因,在变过程中受到表观遗传调节.
- 缺少WFDC15A会影响精子生成,特别是影响精子长.
- 缺少WFDC15A会破坏丸蛋白酶-抗蛋白酶平衡,诱导与高TNFα相似的炎症.
结论:
- WFDC15A对于正常的精子生成和男性生育能力至关重要.
- 一个独特的表观遗传程序控制了WFDC15A的表达,将其与天生的免疫调节联系起来.
- 调节不当的WFDC15A通过受损的精子生成和丸炎症导致男性不育.
相关概念视频
Canonical Wnt Signaling Pathway
8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Dosage Compensation
6.1K
In animals, gender is determined by the number and type of sex chromosome. For example, human females have two X chromosomes, and males have one X and one Y chromosome, whereas C.elegans with one X chromosome is a male, and the one with two X chromosomes is a hermaphrodite.
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will...
6.1K
The Y Chromosome Determines Maleness
6.5K
The Y chromosome is a sex chromosome found in several vertebrates and mammals, including humans. In addition to 22 pairs of autosomes, the human males have one X chromosome and one Y chromosome. In these organisms, the presence or absence of the Y chromosome determines the development of male traits.
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
Evolution
Around 300 million years ago, the two sex chromosomes diverged from two identical autosomal chromosomes. Over time, the Y chromosome has lost most of its genes, shrinking in size....
6.5K
Protein Complexes with Interchangeable Parts
2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K


