Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

pre-mRNA Processing02:01

pre-mRNA Processing

49.0K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
49.0K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K
RNA Editing02:23

RNA Editing

8.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
8.3K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

14.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
14.2K
Epigenetic Regulation01:37

Epigenetic Regulation

3.5K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The Arabidopsis ARF3-AIP1/2-SAP18 module specifies the root stem cell niche in response to auxin.

The Plant cell·2026
Same author

ZmSSRP1-mediated chromatin accessibility modulates maize development via regulation of ZmBRD1 transcription.

Plant physiology·2026
Same author

The TaNHLP1-TaRACK1A module regulates tillering via abscisic acid signaling in wheat.

Nature communications·2025
Same author

Uncovering chromatin accessibility dynamics in early maize endosperm and seed coat differentiation.

The Plant journal : for cell and molecular biology·2025
Same author

LEC2 induces somatic cell reprogramming through epigenetic activation of plant cell totipotency regulators.

Nature communications·2025
Same author

The chromatin accessibility landscape during early maize seed development.

The Plant journal : for cell and molecular biology·2025

相关实验视频

Updated: Apr 30, 2026

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K

玉米核发育的N6-甲基氨酸转录组范围的概况.

Jia Wen Wu1, Guang Ming Zheng1, Lin Zhang1

  • 1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong 271018, China.

Plant physiology
|September 2, 2024
PubMed
概括

N6-甲基氨酸修饰通过影响基因表达来调节玉米核的发育. 这项研究揭示了一个新的转录后表观遗传机制,对玉米育种至关重要.

更多相关视频

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K

相关实验视频

Last Updated: Apr 30, 2026

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K
Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development
10:08

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

9.5K
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
07:18

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

7.4K

科学领域:

  • 植物生物学 植物生物学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 分子生物学分子生物学

背景情况:

  • 玉米核的发育涉及复杂的细胞分裂和分化.
  • 表观遗传修饰,如DNA甲基化,是植物发育的关键调节者.
  • N6-甲基氨酸 (m6A) 是植物中广泛存在的转录后表观遗传修饰,但其在玉米核发育中的作用尚未被探索.

研究的目的:

  • 调查N6-甲基氨酸修饰在玉米核发育中的作用.
  • 在早期的玉米核发育过程中对转录组范围的m6A修改模式进行概述.
  • 确定与玉米核中的m6A修饰相关的基因和调控蛋白.

主要方法:

  • 使用MeRIP-seq和RNA-seq.进行全转录组分析.
  • 分析m6A峰值分布,相关动机和与基因表达的相关性.
  • 遗传学分析来预测m6A调节蛋白.

主要成果:

  • 在玉米核发育的各个阶段 (授粉后0-12天) 确定了数千个m6A峰值.
  • m6A修改主要位于3'-UTR中,与UGUACA动机相关.
  • m6A修饰与核发育过程中无处不在的基因表达的mRNA丰富度负相关.
  • 预测了几种参与m6A修饰和玉米核发育的蛋白质.

结论:

  • N6-甲基氨酸修饰代表了玉米核发育中的新型后转录表观遗传调节机制.
  • 这一发现为玉米的分子育种策略提供了新的见解.
  • 这项研究为进一步研究作物发育中的m6A调节提供了基础.