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相关概念视频

Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
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Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
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相关实验视频

Updated: Jun 14, 2025

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
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Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

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植物组织的组织学方法.

Sheila Criswell1, Brian Gaylord2, Christopher R Pitzer3

  • 1Department of Diagnostic and Health Sciences, University of Tennessee Health Science Center, Memphis, TN, USA.

Journal of histotechnology
|September 2, 2024
PubMed
概括
此摘要是机器生成的。

植物组织的组织学技术可以通过微小的时间调整从动物组织加工中适应. 与植物组织的冷部分相比,固定在甲中的嵌入式 (FFPE) 段提供了优越的形态.

关键词:
细胞壁上的细胞壁.组织学 组织学 组织学叶子 叶子 叶子 叶子形态学 形态学 形态学沙夫兰素 O 是一种沙夫兰素.树干 茎 树干 茎植物生长 植物生长树木 树木 树木 树木

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科学领域:

  • 植物生物学 植物生物学
  • 历史学 历史学 历史学
  • 比较解剖学的比较解剖学.

背景情况:

  • 植物和动物细胞共享有器官,但有很大的差异,需要修改组织学技术.
  • 传统的植物组织加工是耗时的,使用的试剂与动物组织加工不同.

研究的目的:

  • 调查植物组织组织学方面的挑战.
  • 为植物组织适应现代哺乳动物组织加工和染色技术.
  • 为了比较植物样本的FFPE和冷切割方法.

主要方法:

  • 从各种植物中收集的植物器官 (叶子,茎,根,花/果).
  • 使用甲用于抛嵌入 (FFPE) 和甲用于冷部分的加工样本.
  • 在新鲜样本上进行了立即冷切割.
  • 比较FFPE和冷截面的形态和染色特性.
  • 评估了用于植物组织加工的标准哺乳动物组织学试剂和协议.
  • 研究了沙夫兰素O染色加速.

主要成果:

  • 与冷部分相比,固定在甲中嵌入式抛 (FFPE) 部分产生的形态质量优于冷部分.
  • 植物组织的冷部分很难获得,并且在染色过程中表现出组织损失.
  • 现代哺乳动物组织处理协议可以在试剂时间稍微修改后应用于植物组织.
  • 在60°C的温度下化沙夫兰素O显著加快了植物细胞壁的染色.

结论:

  • FFPE提供了一种高质量的植物组织形态学的可靠方法.
  • 标准组织学实验室协议可以适应植物组织处理,减少时间和复杂性.
  • 优化染色协议,如加速沙夫兰素O吸收,增强植物组织分析.