植物组织的组织学方法
Sheila Criswell1, Brian Gaylord2, Christopher R Pitzer3
1Department of Diagnostic and Health Sciences, University of Tennessee Health Science Center, Memphis, TN, USA.
Journal of histotechnology
|September 2, 2024
概括
植物组织的组织学技术可以通过微小的时间调整从动物组织加工中适应. 与植物组织的冷部分相比,固定在甲中的嵌入式 (FFPE) 段提供了优越的形态.
科学领域:
- 植物生物学 植物生物学
- 历史学 历史学 历史学
- 比较解剖学的比较解剖学.
背景情况:
- 植物和动物细胞共享有器官,但有很大的差异,需要修改组织学技术.
- 传统的植物组织加工是耗时的,使用的试剂与动物组织加工不同.
研究的目的:
- 调查植物组织组织学方面的挑战.
- 为植物组织适应现代哺乳动物组织加工和染色技术.
- 为了比较植物样本的FFPE和冷切割方法.
主要方法:
- 从各种植物中收集的植物器官 (叶子,茎,根,花/果).
- 使用甲用于抛嵌入 (FFPE) 和甲用于冷部分的加工样本.
- 在新鲜样本上进行了立即冷切割.
- 比较FFPE和冷截面的形态和染色特性.
- 评估了用于植物组织加工的标准哺乳动物组织学试剂和协议.
- 研究了沙夫兰素O染色加速.
主要成果:
- 与冷部分相比,固定在甲中嵌入式抛 (FFPE) 部分产生的形态质量优于冷部分.
- 植物组织的冷部分很难获得,并且在染色过程中表现出组织损失.
- 现代哺乳动物组织处理协议可以在试剂时间稍微修改后应用于植物组织.
- 在60°C的温度下化沙夫兰素O显著加快了植物细胞壁的染色.
结论:
- FFPE提供了一种高质量的植物组织形态学的可靠方法.
- 标准组织学实验室协议可以适应植物组织处理,减少时间和复杂性.
- 优化染色协议,如加速沙夫兰素O吸收,增强植物组织分析.
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