CYP4CE1 通过两种类型的氧化反应,氧化和N-脱甲基化代谢尼皮拉姆
Huihui Zhang1, Zhen Zhang1, Yixi Zhang1
1Key laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Journal of agricultural and food chemistry
|September 2, 2024
概括
CYP4CE1是昆虫对尼皮拉姆耐药性的关键,通过基化代谢它. CYP6ER1以不同的方式代谢nitenpyram,产生N-desmethyl nitenpyram,突出了P450在抗昆虫剂抵抗中的独特作用.
科学领域:
- 生物化学 生物化学
- 抗虫剂耐药性 抗虫剂耐药性 抗虫剂耐药性
- 分子生物学分子生物学
背景情况:
- 尼皮拉姆是一种新类杀虫剂,用于亚洲的Nilaparvata lugens.
- 细胞染色体P450酶,特别是CYP4CE1和CYP6ER1,与对尼皮拉姆和伊米达克洛普里德的代谢耐药性有关.
- CYP4CE1的表达与尼皮拉姆耐药性相关,而CYP6ER1与伊米达克洛普里德耐药性有关.
研究的目的:
- 调查CYP4CE1在尼皮拉姆代谢中的特定作用.
- 通过CYP4CE1.1阐明尼皮拉姆的代谢途径和产物.
- 为了比较CYP4CE1和CYP6ER1与nitenpyram的代谢能力.
主要方法:
- 在现场采集的昆虫种群中分析CYP4CE1和CYP6ER1基因表达.
- 质谱测量用于识别尼皮拉姆的代谢物.
- 在体外代谢测定使用重组CYP4CE1和CYP6ER1酶.
主要成果:
- 在现场种群中,CYP4CE1表达与尼皮拉姆耐药性密切相关.
- 再组合CYP4CE1将尼皮拉姆代谢成N-脱甲基尼皮拉姆,氧-尼皮拉姆和N-脱甲基氧-尼皮拉姆,偏好氧化.
- 在CYP6ER1的代谢过程中,nitenpyram只会转化为N-desmethyl nitenpyram.
结论:
- 通过氧化,CYP4CE1在耐尼皮拉姆耐药性方面发挥着特定的作用.
- 不同的P450酶表现出不同的基质特异性和子类代谢中的催化机制.
- 了解这些特定的代谢途径对于管理抗虫剂耐药性至关重要.
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