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¹³C标记的葡萄糖揭示了在混合微生物培养的阴极电发酵过程中发酵途径的变化

Gaia Salvatori¹, Ottavia Giampaoli^2,3, Angela Marchetti¹

¹Department of Chemistry, Sapienza University of Rome, P.le Aldo Moro 5, 00185, Rome, Italy.

|September 2, 2024

概括

阴极电发酵 (CEF) 通过改变代谢途径来控制发酵产品. 这种方法提高了从葡萄糖中获得的酸盐,酸盐和丁酸盐产量,提供了一个新的生物过程控制工具.

科学领域:

生物技术和生物工程生物技术和生物工程
微生物的新陈代谢
电化学过程电化学过程

背景情况:

无氧发酵产生各种产品,但控制产量是一项挑战.
阴极电发酵 (CEF) 是一种管理发酵结果的新兴技术.
了解CEF对代谢途径的影响对于过程优化至关重要.

研究的目的:

用混合微生物培养物研究阴极极化在葡萄糖发酵中的作用.
在CEF条件下使用13C标记的葡萄糖分析葡萄糖发酵的代谢途径.
量化CEF对关键发酵产品生产的影响.

主要方法:

使用混合微生物培养,使用标记13C的葡萄糖和未标记的酸盐/乙醇的三元混合物.
应用了正极电位 (-700 mV与SHE) 并将结果与开放电路电位 (OCP) 控制器进行了比较.
分析了13C标记的发酵产品及其微分丰富物,以建模代谢途径.

主要成果:

与OCP对照组相比,CEF显著提高了酸盐,酸盐和丁酸盐的生产产量.
CEF改变了13C标记的发酵产品含量和分量缩,使代谢途径得到阐明.
在阴极极化下观察到增加了完全标记13C的propionate和butyrate同位素的丰度.

结论:

在CEF中,阴极极化通过影响中介产物而影响葡萄糖代谢途径.
CEF为控制混合微生物培养生物工艺和产品产量提供了一个有希望的策略.
这项研究促进了对CEF机制及其在生物技术中的潜在应用的理解.

关键词:

阴极电发酵 (CEF) 是一种完全标记的葡萄糖 (U-13C)混合微生物培养 (MMC)核磁共振 (NMR) 是一种核磁共振技术.短链碳酸 (SCCA) 是一种

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