兰塔纳卡马拉L.诱导了莱什马尼亚亚马逊菌的多目标细胞死亡过程
Ari Sérgio de Oliveira Lemos1, Juliana da Trindade Granato1, Luciana Maria Ribeiro Antinarelli1
1Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil.
Journal of ethnopharmacology
|September 2, 2024
概括
兰塔纳卡玛拉提取物显示出强大的抗莱什马尼亚抗莱什马尼亚亚马逊菌的活性,通过多种细胞死亡途径起作用. 这项研究强调了其作为莱什曼病的新型治疗剂的潜力.
科学领域:
- 植物化学 植物化学
- 寄生虫学的寄生虫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 兰塔纳卡马拉 (Lantana camara L.) 传统上用于各种疾病,并具有多种不同的二次代谢产物.
- 尽管它具有丰富的化学特征,但对其抗莱什曼特性的研究是有限的.
研究的目的:
- 为了调查Lantana camara乙醇提取物 (LCE) 对Leishmania amazonensis的抗莱什曼病毒潜力.
- 为了阐明LCE的作用机制.
主要方法:
- 准备和植物化学分析 (UFLC-QTOF-MS) 的LCE.
- 使用MTT测定和Giemsa染色,评估LCE对Leishmania amazonensis前列腺菌和细胞内前列腺菌的影响.
- 对小鼠巨细胞的细胞毒性评估和通过SEM,TEM和生物化学测试对细胞死亡机制的调查.
主要成果:
- LCE显示出显著的抗莱什曼尼活性,低IC50值对前列腺炎和前列腺炎,与miltefosine相似.
- 提取物具有很高的选择性,对巨细胞的细胞毒性可以忽略不计 (SI = 31.50).
- LCE诱导多目标细胞死亡,涉及氧化应激,线粒体功能障碍和等离子体膜变化,与被确定为主要成分的类类类类.
结论:
- 兰塔纳卡玛拉提取物富含烯,通过结合的细胞亡和非细胞亡细胞死亡机制表现出有前途的抗莱什曼细胞活性.
- 作为一种新型抗莱什曼药物开发的候选药物,LCE具有潜力.
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