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对刺信号通路的分子洞察力与急性淋巴细胞白血病的非编码RNA相关,这是一个生物信息学研究

Elham Talebi¹, Pegah Ghoraeian², Zinat Shams³

¹Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Annals of hematology

|September 2, 2024

概括

这项研究揭示了关键的长非编码RNA (lncRNAs) 与急性淋巴细胞白血病 (ALL) 中的 Hedgehog (Hh) 信号相互作用. 这些发现表明,对于ALL患者来说,潜在的新诊断生物标志物和治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

急性淋巴细胞白血病 (ALL) 是一种常见的血液性恶性瘤,结果各异.
分子生物学的进步突出了 (Hh) 信号和非编码RNA (ncRNA) 在ALL中的作用.
了解Hh信号与ncRNA之间的相互作用对于ALL研究至关重要.

研究的目的:

研究连接 (Hh) 信号和急性淋巴细胞白血病 (ALL) 的分子机制.
确定与ALL中Hh信号通路基因相互作用的长非编码RNA (lncRNAs).
探索这些相互作用作为诊断生物标志物和治疗点的潜力.

主要方法:

利用系统生物学方法重新分析基因表达综合 (GEO) 微阵列数据,用于ALL中的差异表达基因 (DEGs) 和lncRNAs.
应用了皮尔森的相关性分析来确定Hh信号通路基因和差异表达的lncRNAs (DElncRNAs) 之间的关系.
通过识别向Hh信号通路相关的信使RNA (mRNA) 的微RNA (miRNA) 来构建一个调控网络.

主要成果:

在ALL和正常骨髓样本之间确定了193个DEG和226个DElncRNA.
发现了与Hh信号通路相关的DEGs和26个DElncRNAs之间的显著相关性.
观察到DElncRNAs与关键生物过程之间的联系,包括耐药性和免疫调节. 在 Hh 途径 DEG 和已知的 ALL 相关的 miRNA 之间确定了共享的 miRNA (例如,miR-155-5p,miR-211).

结论:

在ALL的背景下,阐明了Hh信号,lncRNA和miRNA之间的复杂相互作用.
突出了与Hh信号通路基因相关的DElncRNAs作为ALL新型诊断生物标志物的潜力.
建议这些DElncRNAs可以作为急性淋巴细胞白血病的有前途的治疗点.

关键词:

急性淋巴细胞白血病 (Acute Lymphoblastic Leukemia) 是一种急性淋巴细胞白血病.刺信号通道的信号通道皮尔森的相关性分析分析.在 lncRNAs 中.这就是miRNAs.

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