在不同口腔潜在恶性疾病和状细胞癌中,殖民微生物景观的差异:一个多组比较研究
Xuan Zhou1,2, Xinjia Cai1,2, Qian Tang3
1Department of Oral Pathology, National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices& Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, No.22, Zhongguancun South Avenue, Haidian District, Beijing, 100081, PR China.
BMC microbiology
|September 2, 2024
概括
口腔潜在恶性疾病 (OPMDs) 显示出不同的微生物群落. 口腔白血病 (OLK) 具有很高的微生物丰富性,而PVL-SCC类似于健康的对照,表明口腔微生物群在OPMD进展中的独特作用.
科学领域:
- 微生物学 微生物学
- 口腔健康 口腔健康
- 在瘤学瘤学.
背景情况:
- 人们越来越认识到微生物在疾病,特别是癌症中的作用.
- 在口腔潜在恶性疾病 (OPMDs) 中口腔微生物群的研究是有限的.
- 这项研究的重点是描述各种OPMD中的微生物群落.
研究的目的:
- 研究和比较不同OPMD中的微生物群落.
- 识别与特定OPMD类型相关的独特微生物特征.
- 探索在增殖性白性白血病向状细胞癌 (PVL-SCC) 的进展过程中的微生物变化.
主要方法:
- 分析了患有口腔白血病 (OLK),增殖性性白血病 (PVL),口腔平面 (OLP),口腔状病变 (OLL) 和PVL-SCC的患者的口腔活检.
- 从巴拉芬嵌入组织中提取DNA,然后进行2bRAD-M测序以进行分类分析.
- 进行了α和β多样性分析和线性差异分析效果大小 (LEfSe) 来比较微生物组成.
主要成果:
- 在OPMD组中观察到微生物丰富性和多样性的显著差异.
- PVL-SCC表现出与健康对照组相似的微生物概况,而OLK显示出最高的丰富度.
- 在PVL-SCC进展过程中,每个疾病群体都有不同的主导细菌物种,显着的变化包括减少Fusobacterium periodonticum和增加Prevotella oris.
结论:
- 每个OPMD群体都具有独特的口腔微生物群组成和独特的主导细菌物种.
- 这些微生物差异可能在OPMDs的发病过程中发挥作用.
- 了解这些独特的微生物特征,可以了解疾病机制.
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