探索光学活性溶剂在基拉色谱中对基于多糖的柱子的影响
Turaj Rahmani1, Frédéric Lynen1
1Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4, B-9000 Ghent, Belgium.
Analytical chemistry
|September 3, 2024
概括
光学活跃的l-乙基乳酸盐 (LEL) 作为基拉液体染色学 (LC) 中的移动相修饰剂具有前景,为某些化合物提供独特的选择性和更好的分辨率. 这种生物基溶剂增强了性分离,并简化了选平台.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色体学.
- 有机化学 有机化学
背景情况:
- 嵌合体液体染色学 (LC) 依赖于移动相修饰器来调整嵌合体选择性.
- 光学活性溶剂提供了一种新的方法来增强形识别.
- 像L-乙烯乳酸盐 (LEL) 这样的生物基溶剂提供了可持续的替代品.
研究的目的:
- 调查l-乙烯乳酸盐 (LEL) 作为手动相修饰剂在合性LC中的有效性.
- 为了比较LEL的性能与常见的阿基拉溶剂,如乙二 (ACN) 和甲醇 (MeOH).
- 为了探索性溶剂相互作用对enantioseparations的影响.
主要方法:
- 用LEL作为ACN和MeOH的部分或完全替代品进行了逆相LC (RPLC) 奇拉分离.
- 在多糖柱上测试了各种条件.
- 对d-乙基乳酸 (DEL) 的影响与LEL进行了比较,以研究性相互作用.
主要成果:
- 在16种测试化合物中,LEL表现出明显的性选择性,改善了8种化合物的分辨率.
- LEL与ACN比MeOH表现更好,这可能是由于甲醇的原始性质.
- 与ACN相比,LEL在较低的温度下呈现出更的奇拉分辨率增加,并且与盐添加剂具有很好的兼容性.
结论:
- 光学活性的l-乙基乳酸盐 (LEL) 是一种可行的和有效的移动相修饰器,用于合性LC.
- LEL提供了独特的选择性,并可以简化奇拉性选平台,促进更绿色的分析方法.
- 溶剂和分析物之间的基拉尔相互作用显著影响了反分离,通过比较LEL和DEL来证明这一点.
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