牙干细胞复合支架在动物裂口口腔模型中的骨质效应
Kasem Rattanapinyopituk1, Chaiyapol Chaweewannakorn2, Nathaphon Tangjit2
1Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Heliyon
|September 3, 2024
概括
在PCL-GE和PLGA-BG支架中,来自人体脱皮脱落牙 (SHED) 的干细胞在老鼠裂缺陷中促进骨再生. 用SHED增强的脚手架显示了对裂口修复的强化骨质生成功效.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 裂口口腔缺陷会带来重大的重建性挑战.
- 生物材料支架对于面缺陷的骨再生至关重要.
- 干细胞有望促进骨愈合.
研究的目的:
- 在两个复合支架内评估来自人体脱皮叶状牙 (SHED) 的干细胞的骨质生成潜力.
- 在大鼠裂口模型中,评估聚烯酸与凝 (PCL-GE) 和聚-乳糖-糖酸) -生物活性玻璃 (PLGA-BG) 支架的有效性.
- 为了确定SHED是否在纳入这些支架时增强骨再生.
主要方法:
- 在Sprague-Dawley大鼠中,形成了类似口腔裂的缺陷.
- 鼠接受了PCL-GE或PLGA-BG支架,有或没有SHED.
- 植入后2,3个月和6个月进行了组织学和免疫组织化学分析.
主要成果:
- 与对照组相比,PCL-GE和PLGA-BG支架都促进了骨的形成.
- 与PCL-GE脚手架相比,PLGA-BG脚手架表现出优异的骨再生.
- 结合SHED的支架显著加速了骨的形成,并显示了人类细胞的存在.
结论:
- PCL-GE和PLGA-BG复合支架在裂缺陷中有效修复骨组织.
- 补充SHED可以增强这些生物材料支架的骨质生效效.
- 这些SHED增强的支架代表了裂口修复和骨组织工程的有希望的候选人.
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