一种可靠的方法来准备毫克大小的环境样本,用高分辨率石墨炉原子吸收光谱测量量金属的含量
Patrícia Gomes Costa1, Juliana Lemos Dal Pizzol1, Yuri Dornelles Zebral1
1Instituto de Ciências Biológicas, Universidade Federal do Rio Grande - FURG, Avenida Itália km 8, Campus Carreiros, 96.203-900, Rio Grande, RS, Brazil.
MethodsX
|September 3, 2024
概括
一种使用酸消化在干燥炉中的新方法精确地量化了小样本中的金属化物. 这种高效的技术非常适合环境监测,降低成本和使用危险化学品.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
背景情况:
- 准确量化金属化物对于环境监测至关重要.
- 分析毫克大小样本的现有方法可能耗时且资源密集.
研究的目的:
- 确定一种精确而高效的提取方法,用于在毫克尺寸的生物和沉积物样本中量化金属.
- 为了验证这种方法,使用高分辨率石墨炉原子吸收光谱学 (HR-GFAAS).
主要方法:
- 经过认证的参考材料 (生物:DORM-4,DOLT-5,TORT-3;沉积物:MESS-4) 的消化,使用酸在干炉中,用皇家水在干炉中,或用酸在微波炉中.
- 对酸和酸混合物的评估,用于沉积物消化.
- 对抽取效率的样本大小 (100 mg 和 200 mg) 的评估.
主要成果:
- 在干燥炉中的酸消化实现了所有经过测试的认证参考材料中金属化物的最高回收率 (80.0%-100.0%).
- 其他测试方法的恢复率较低 (67.3%-99.2%).
- 样本大小通常不会显著影响提取效率.
结论:
- 在干燥炉中消化酸是一种可靠和有效的方法,用于从毫克大小的环境样本中提取金属 (化物) 来进行HR-GFAAS分析.
- 这种经过验证的方法通过减少时间,成本和危险化学品的使用,为环境质量监测提供了显著的好处.
- 该方法提供了一种简单,可靠的方法,用于分析小型环境样本的金属和化物.
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