昂科斯塔丁M促进了表皮屏障功能障碍在患有带有鼻多的eosinophilic慢性鼻炎的患者
Bao-Feng Wang1, Ying-Ying Wang1, Hai Lin2
1Department of Otolaryngology-Head and Neck Surgery, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua University, Beijing, P.R. China.
Tissue barriers
|September 3, 2024
概括
哥斯塔丁M (OSM) 在鼻细胞中提升矩阵金属蛋白酶 (MMP) -1 和 -7,有助于在带有鼻息肉的eosinophilic慢性鼻炎 (Eos CRSwNP) 中的屏障功能障碍. 这突出了OSM的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 带有鼻的eosinophilic慢性鼻炎 (Eos CRSwNP) 与粘膜表皮屏障功能障碍有关.
- 昂哥斯塔丁M (OSM) 通过诱导矩阵金属蛋白酶 (MMPs) 来促进这种功能障碍.
研究的目的:
- 研究OSM在调节鼻上皮细胞 (NEC) 中MMP-1和MMP-7合成中的作用和机制.
- 在Eos CRSwNP的背景下,评估OSM对上皮屏障的影响.
主要方法:
- 使用定量聚合酶链反应 (qPCR),免疫光和免疫组织化学来评估OSM,OSMR,MMP-1和MMP-7在鼻子组织和NEC中的表达.
- 通过OSM和细胞因子刺激初级NEC和空气液体接口 (ALI) 培养物.
- 使用ELISA和免疫光测试来测量蛋白质表达和紧结蛋白 (occludin) 局部化.
主要成果:
- 与对照组相比,在Eos CRSwNP组织和NEC中观察到OSMRβ,MMP-1和MMP-7水平升高.
- 在NEC中,OSM刺激显著增加了MMP-1和MMP-7的表达,而这种表达被STAT3和PI3K抑制剂抑制.
- OSM刺激破坏了NEC中的奥克卢丁局部化,表明受损的紧接口,这种效应被泛MMP抑制剂逆转.
结论:
- 在NEC中,OSM诱导MMP-1和MMP-7的合成和释放.
- 在Eos CRSwNP患者观察到的粘膜上皮屏障功能障碍中,MMP-1和MMP-7起着至关重要的作用.
- 针对OSM或MMP可能为Eos CRSwNP提供治疗策略.
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